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Literature summary for 3.2.1.4 extracted from

  • Li, W.; Ji, P.; Zhou, Q.; Hua, C.; Han, C.
    Insights into the synergistic biodegradation of waste papers using a combination of thermostable endoglucanase and cellobiohydrolase from Chaetomium thermophilum (2018), Mol. Biotechnol., 60, 49-54 .
    View publication on PubMed
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of C-termially His6-tagged enzyme in Pichia pastoris strain GS115 Thermochaetoides thermophila

Inhibitors

Inhibitors Comment Organism Structure
Ag+ slight inhibition at 10 mM Thermochaetoides thermophila
Al3+ slight inhibition at 10 mM Thermochaetoides thermophila
Co2+ activates 38% at 2 mM, inhibits 20% at 10 mM Thermochaetoides thermophila
Cr3+
-
 Thermochaetoides thermophila
Cu2+ complete inhibition at 2-10 mM Thermochaetoides thermophila
Fe3+ complete inhibition at 2-10 mM Thermochaetoides thermophila
Ni2+ activates 8% at 2 mM, inhibits 8% at 10 mM Thermochaetoides thermophila
Pb2+
-
 Thermochaetoides thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Thermochaetoides thermophila
Co2+ activates 38% at 2 mM, inhibits 20% at 10 mM Thermochaetoides thermophila
Mn2+ activates Thermochaetoides thermophila
Sr2+ activates Thermochaetoides thermophila
Zn2+ activates Thermochaetoides thermophila

Organism

Organism UniProt Comment Textmining
Thermochaetoides thermophila G0S9G3
-
-
Thermochaetoides thermophila CBS 144.50 G0S9G3
-
-
Thermochaetoides thermophila DSM 1495 G0S9G3
-
-
Thermochaetoides thermophila IMI 039719 G0S9G3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Pichia pastoris strain GS115 by nickel affinity chromatography Thermochaetoides thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
 Thermochaetoides thermophila ?
-
 ?
carboxymethyl cellulose + H2O
-
 Thermochaetoides thermophila IMI 039719 ?
-
 ?
carboxymethyl cellulose + H2O
-
 Thermochaetoides thermophila DSM 1495 ?
-
 ?
carboxymethyl cellulose + H2O
-
 Thermochaetoides thermophila CBS 144.50 ?
-
 ?
additional information synergistic biodegradation of waste papers using a combination of thermostable endoglucanase CTendo45 and cellobiohydrolase CtCel6 from Chaetomium thermophilum. CtCel6 significantly enhances the bioconversion process, and CTendo45 synergistically increases the degradation, with a maximum degree of synergistic effect of 1.67 when the mass ratio of CTendo45/CtCel6 is 5:3. Enzymatic hydrolysis of different paper materials, e.g. filter paper and office paper giving high activity, or newspaper and cardboard resulting in low activity, foolscap paper gives moderate activity. For newspaper, the recalcitrance to the enzymatic action may be caused by the high contents of hemicelluloses, lignin and various inorganic components Thermochaetoides thermophila ?
-
 ?
additional information synergistic biodegradation of waste papers using a combination of thermostable endoglucanase CTendo45 and cellobiohydrolase CtCel6 from Chaetomium thermophilum. CtCel6 significantly enhances the bioconversion process, and CTendo45 synergistically increases the degradation, with a maximum degree of synergistic effect of 1.67 when the mass ratio of CTendo45/CtCel6 is 5:3. Enzymatic hydrolysis of different paper materials, e.g. filter paper and office paper giving high activity, or newspaper and cardboard resulting in low activity, foolscap paper gives moderate activity. For newspaper, the recalcitrance to the enzymatic action may be caused by the high contents of hemicelluloses, lignin and various inorganic components Thermochaetoides thermophila IMI 039719 ?
-
 ?
additional information synergistic biodegradation of waste papers using a combination of thermostable endoglucanase CTendo45 and cellobiohydrolase CtCel6 from Chaetomium thermophilum. CtCel6 significantly enhances the bioconversion process, and CTendo45 synergistically increases the degradation, with a maximum degree of synergistic effect of 1.67 when the mass ratio of CTendo45/CtCel6 is 5:3. Enzymatic hydrolysis of different paper materials, e.g. filter paper and office paper giving high activity, or newspaper and cardboard resulting in low activity, foolscap paper gives moderate activity. For newspaper, the recalcitrance to the enzymatic action may be caused by the high contents of hemicelluloses, lignin and various inorganic components Thermochaetoides thermophila DSM 1495 ?
-
 ?
additional information synergistic biodegradation of waste papers using a combination of thermostable endoglucanase CTendo45 and cellobiohydrolase CtCel6 from Chaetomium thermophilum. CtCel6 significantly enhances the bioconversion process, and CTendo45 synergistically increases the degradation, with a maximum degree of synergistic effect of 1.67 when the mass ratio of CTendo45/CtCel6 is 5:3. Enzymatic hydrolysis of different paper materials, e.g. filter paper and office paper giving high activity, or newspaper and cardboard resulting in low activity, foolscap paper gives moderate activity. For newspaper, the recalcitrance to the enzymatic action may be caused by the high contents of hemicelluloses, lignin and various inorganic components Thermochaetoides thermophila CBS 144.50 ?
-
 ?

Synonyms

Synonyms Comment Organism
CTendo45
-
 Thermochaetoides thermophila
ctendo7
-
 Thermochaetoides thermophila
CTHT_0045780 gene name, UniProt Thermochaetoides thermophila
endoglucanase
-
 Thermochaetoides thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 in vivo assay at Thermochaetoides thermophila