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Literature summary for 3.2.1.4 extracted from
- The unique GH5 cellulase member in the extreme halotolerant fungus Aspergillus glaucus CCHA is an endoglucanase with multiple tolerance to salt, alkali and heat prospects for straw degradation applications (2018), Extremophiles, 22, 675-685 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| sequence comparisons, recombinant expression of His-tagged enzyme in Pichia pastoris strain GS115 | Aspergillus glaucus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Aspergillus glaucus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | activates about 20% at 1 mM | Aspergillus glaucus | |
| Ca2+ | activates about 20% at 1 mM | Aspergillus glaucus | |
| Co2+ | activates 2fold at 1 mM | Aspergillus glaucus | |
| Cu2+ | activates 1.5fold at 1 mM | Aspergillus glaucus | |
| Mg2+ | activates about 30% at 1 mM | Aspergillus glaucus | |
| Mn2+ | activates about 20% at 1 mM | Aspergillus glaucus | |
| NaCl | the enzyme shows higher activity at NaCl concentrations from 1.0 to 4.0 M | Aspergillus glaucus | |
| Ni2+ | activates about 20% at 1 mM | Aspergillus glaucus | |
| Zn2+ | activates about 25% at 1 mM | Aspergillus glaucus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus glaucus | A0A2P1G1K5 | i.e. Eurotium herbariorum | - |
| Aspergillus glaucus CCHA | A0A2P1G1K5 | i.e. Eurotium herbariorum | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme has two potential N-glycosylation sites at the N-terminus (positions 37 and 60) and 60 potential O-glycosylation sites at the C-terminus | Aspergillus glaucus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Pichia pastoris strain GS115 by nickel affinity chromatography to homogeneity | Aspergillus glaucus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 297.15 | - |
purified recombinant His-tagged enzyme, pH 5.0, 55°C, substrate carboxymethyl cellulose | Aspergillus glaucus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethyl cellulose + H2O | - |
Aspergillus glaucus | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Aspergillus glaucus CCHA | ? | - |
? | |
| additional information | hydrolysis of rice and corn straws | Aspergillus glaucus | ? | - |
? | |
| additional information | hydrolysis of rice and corn straws | Aspergillus glaucus CCHA | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, about, recombinant enzyme, SDS-PAGE, x * 52800, about, sequence calculation | Aspergillus glaucus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AgCMCase | - |
Aspergillus glaucus |
| endoglucanase | - |
Aspergillus glaucus |
| GH5 cellulase | - |
Aspergillus glaucus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
recombinant enzyme | Aspergillus glaucus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 90 | over 60% of maximal activity within this range, profile overview | Aspergillus glaucus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
recombinant enzyme | Aspergillus glaucus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | over 30% of maximal activity within this range, profile overview | Aspergillus glaucus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | purified recombinant His-tagged enzyme, over 50% activity remaining at pH 4.0-8.5, 40% at pH 9.0-10.0 | Aspergillus glaucus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus glaucus | sequence calculation | - |
5.2 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycosyl hydrolase family 5, GH5 | Aspergillus glaucus |
| additional information | the unique GH5 cellulase member in the extreme halotolerant fungus Aspergillus glaucus CCHA is an endoglucanase with multiple tolerance to salt, alkali and heat. Homology modeling of AgCMCase | Aspergillus glaucus |
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