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Literature summary for 3.2.1.4 extracted from

  • Berto, G.L.; Velasco, J.; Tasso Cabos Ribeiro, C.; Zanphorlin, L.M.; Noronha Domingues, M.; Tyago Murakami, M.; Polikarpov, I.; de Oliveira, L.C.; Ferraz, A.; Segato, F.
    Functional characterization and comparative analysis of two heterologous endoglucanases from diverging subfamilies of glycosyl hydrolase family 45 (2019), Enzyme Microb. Technol., 120, 23-35 .
    View publication on PubMed

Application

Application Comment Organism
industry endoglucanases from family GH45 are applied in formulation of detergents and in industrial pulp and paper processes Gloeophyllum trabeum
industry endoglucanases from family GH45 are applied in formulation of detergents and in industrial pulp and paper processes Thermothelomyces thermophilus

Cloned(Commentary)

Cloned (Comment) Organism
phylogenetic analysis and tree Gloeophyllum trabeum
phylogenetic analysis and tree Thermothelomyces thermophilus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ slight inhibition Gloeophyllum trabeum
Cu2+ strong inhibition Thermothelomyces thermophilus
EDTA slight inhibition Gloeophyllum trabeum
Fe2+ complete inhibition Gloeophyllum trabeum
Fe2+ complete inhibition Thermothelomyces thermophilus
Mg2+ slight inhibition Gloeophyllum trabeum
Mn2+ strong inhibition Thermothelomyces thermophilus
Na+ slight inhibition Gloeophyllum trabeum
Na+ slight inhibition Thermothelomyces thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 50.9% activation Thermothelomyces thermophilus
Co2+ 87.8% activation Gloeophyllum trabeum

Organism

Organism UniProt Comment Textmining
Gloeophyllum trabeum
-
-
-
Thermothelomyces thermophilus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3
-
purified enzyme MtGH45, substrate phosphoric acid swollen cellulose, pH 6.5, 50°C Thermothelomyces thermophilus
3
-
purified enzyme MtGH45, substrate Avicel, pH 6.5, 50°C Thermothelomyces thermophilus
3.3
-
purified enzyme GtGH45, substrate phosphoric acid swollen cellulose, pH 6.5, 50°C Gloeophyllum trabeum
8.5
-
purified enzyme GtGH45, substrate cotton, pH 6.5, 50°C Gloeophyllum trabeum
8.8
-
purified enzyme GtGH45, substrate Avicel, pH 6.5, 50°C Gloeophyllum trabeum
12.8
-
purified enzyme MtGH45, substrate cotton, pH 6.5, 50°C Thermothelomyces thermophilus
35.6
-
purified enzyme MtGH45, substrate lichenan, pH 6.5, 50°C Thermothelomyces thermophilus
52.5
-
purified enzyme MtGH45, substrate beta-glucan, pH 6.5, 50°C Thermothelomyces thermophilus
54.2
-
purified enzyme GtGH45, substrate lichenan, pH 6.5, 50°C Gloeophyllum trabeum
170.7
-
purified enzyme GtGH45, substrate beta-glucan, pH 6.5, 50°C Gloeophyllum trabeum
200.6
-
purified enzyme GtGH45, substrate carboxymethyl cellulose, pH 6.5, 50°C Gloeophyllum trabeum
202.6
-
purified enzyme MtGH45, substrate carboxymethyl cellulose, pH 6.5, 50°C Thermothelomyces thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O
-
Gloeophyllum trabeum ?
-
?
avicel + H2O
-
Thermothelomyces thermophilus ?
-
?
beta-glucan + H2O
-
Gloeophyllum trabeum ?
-
?
beta-glucan + H2O
-
Thermothelomyces thermophilus ?
-
?
carboxymethyl cellulose + H2O best substrate Gloeophyllum trabeum ?
-
?
carboxymethyl cellulose + H2O best substrate Thermothelomyces thermophilus ?
-
?
cellohexaose + H2O
-
Gloeophyllum trabeum D-glucose + ?
-
?
cellohexaose + H2O
-
Thermothelomyces thermophilus cellobiose + cellotriose + cellotetraose
-
?
cellopentaose + H2O
-
Gloeophyllum trabeum D-glucose + ?
-
?
cellopentaose + H2O
-
Thermothelomyces thermophilus cellobiose + cellotriose
-
?
cellotetraose + H2O
-
Gloeophyllum trabeum D-glucose + cellobiose + cellotriose
-
?
cellotriose + H2O
-
Gloeophyllum trabeum D-glucose + cellobiose
-
?
cotton + H2O
-
Gloeophyllum trabeum ?
-
?
cotton + H2O
-
Thermothelomyces thermophilus ?
-
?
lichenan + H2O
-
Gloeophyllum trabeum ?
-
?
lichenan + H2O
-
Thermothelomyces thermophilus ?
-
?
additional information substrate specificity, overview. Enzyme MtGH45 is unable to hydrolyze C3 and C4 oligosaccharides, cellobiose and cellotriose are detected as the main products when the enzyme is incubated with cellopentaose, which is completely hydrolyzed and the incubation with cellohexaose generates cellobiose, cellotriose, and cellotetraose as main products Thermothelomyces thermophilus ?
-
?
additional information substrate specificity, overview. The GtGH45 is able to convert all tested oligosaccharides to glucose, however, small amounts of cellobiose, cellotriose, and cellotetraose are detected in the reaction products, indicating that the enzyme cannot convert efficiently small oligosaccharides probably due their mode of recognition in the enzyme catalytic cleft. The degradation of C5 and C6 by GtGH45 is almost complete with glucose being a main product Gloeophyllum trabeum ?
-
?
phosphoric acid swollen cellulose + H2O
-
Gloeophyllum trabeum ?
-
?
phosphoric acid swollen cellulose + H2O
-
Thermothelomyces thermophilus ?
-
?

Synonyms

Synonyms Comment Organism
endoglucanase
-
Gloeophyllum trabeum
endoglucanase
-
Thermothelomyces thermophilus
GH45 endoglucanase
-
Gloeophyllum trabeum
GH45 endoglucanase
-
Thermothelomyces thermophilus
GtGH45
-
Gloeophyllum trabeum
MtGH45
-
Thermothelomyces thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Gloeophyllum trabeum
65
-
-
Thermothelomyces thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
66
-
melting temperature of enzyme GtGH45 Gloeophyllum trabeum
80.9
-
melting temperature of enzyme MtGH45 Thermothelomyces thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Gloeophyllum trabeum
5
-
-
Thermothelomyces thermophilus

pH Range

pH Minimum pH Maximum Comment Organism
4 6 over 50% of maximal activity within this range Gloeophyllum trabeum
4.5 10 over 50% of maximal activity within this range Thermothelomyces thermophilus

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosyl hydrolase family 45, GH45, subfamily GH45A Thermothelomyces thermophilus
evolution the enzyme belongs to the glycosyl hydrolase family 45, GH45, subfamily GH45C Gloeophyllum trabeum
additional information molecular docking of enzyme GtGH45 with cellooligosaccharides cellopentaose, cellotetraose, cellotriose, and cellobiose Gloeophyllum trabeum
additional information the molecular docking simulations of MtGH45 reveal the requirements of the sites -2, -1, +1, +2 and +3 to be occupied for hydrolysis. Molecular docking of C4, C3 and C2 is unproductive Thermothelomyces thermophilus