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Literature summary for 3.2.1.4 extracted from

  • Daas, M.J.A.; Nijsse, B.; van de Weijer, A.H.P.; Groenendaal, B.W.A.J.; Janssen, F.; van der Oost, J.; van Kranenburg, R.
    Engineering Geobacillus thermodenitrificans to introduce cellulolytic activity; expression of native and heterologous cellulase genes (2018), BMC Biotechnol., 18, 42 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
gene ge40, DNA and amino acid sequence determination and analysis, sequence comparisons, genetic structure of the cloning construct, cloning with a signal peptide sequence derived from the endoxylanse (EC 3.2.1.8) encoding gene GtxynA1 from Geobacillus thermodenitrificans strain T12, overview. Recombinant expression of the enzyme in Escherichia coli strain BL21(DE3) (under control of the lac operator) and in Geobacillus thermodenitrificans strain T12 Geobacillus thermodenitrificans

Organism

Organism UniProt Comment Textmining
Geobacillus thermodenitrificans A0A291I5R3
-
-
Geobacillus thermodenitrificans T12 A0A291I5R3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxyethyl cellulose + H2O
-
 Geobacillus thermodenitrificans ?
-
 ?
2-hydroxyethyl cellulose + H2O
-
 Geobacillus thermodenitrificans T12 ?
-
 ?
barley beta-glucan + H2O
-
 Geobacillus thermodenitrificans ?
-
 ?
barley beta-glucan + H2O
-
 Geobacillus thermodenitrificans T12 ?
-
 ?
carboxymethyl cellulose + H2O
-
 Geobacillus thermodenitrificans ?
-
 ?
carboxymethyl cellulose + H2O
-
 Geobacillus thermodenitrificans T12 ?
-
 ?

Synonyms

Synonyms Comment Organism
endoglucanase
-
 Geobacillus thermodenitrificans
GE40
-
 Geobacillus thermodenitrificans
GE40 endoglucanase
-
 Geobacillus thermodenitrificans
T12-GE40
-
 Geobacillus thermodenitrificans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Geobacillus thermodenitrificans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Geobacillus thermodenitrificans

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycoside hydrolase family 5, GH5 Geobacillus thermodenitrificans