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Literature summary for 3.2.1.4 extracted from
- Isolation and characterization of a non-specific endoglucanase from a metagenomic library of goat rumen (2016), World J. Microbiol. Biotechnol., 32, 1-8 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | uncultured microorganism |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
uncultured microorganism |  |
| EDTA | - |
uncultured microorganism | |
| Mg2+ | - |
uncultured microorganism | |
| Ni2+ | - |
uncultured microorganism | |
| SDS | - |
uncultured microorganism | |
| Zn2+ | - |
uncultured microorganism | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
carboxymethylcellulose | Km value 0.144 mg/ml, pH 5.0, temperature not specified in the publication | uncultured microorganism | |
| additional information | - |
oat spelt xylan | Km value 2.75 mg/ml, pH 5.0, temperature not specified in the publication | uncultured microorganism | |
| additional information | - |
birchwood xylan | Km value 4.37 mg/ml, pH 5.0, temperature not specified in the publication | uncultured microorganism |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
uncultured microorganism | |
| Co2+ | - |
uncultured microorganism | |
| Fe2+ | - |
uncultured microorganism | |
| Mn2+ | - |
uncultured microorganism | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured microorganism | X5CIW4 | rumen microbial metagenome library of goat rumen microorganisms | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| birchwood xylan + H2O | 44% of the activity with carboxymethylcellulose | uncultured microorganism | ? | - |
? | |
| carboxymethylcellulose + H2O | - |
uncultured microorganism | ? | - |
? | |
| additional information | poor activity against filter paper and avicel | uncultured microorganism | ? | - |
? | |
| oat spelt xylan + H2O | 59% of the activity with carboxymethylcellulose | uncultured microorganism | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, calculated including His-tag | uncultured microorganism |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cel28a | - |
uncultured microorganism |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
uncultured microorganism |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
uncultured microorganism |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| uncultured microorganism | calculated | - |
5.2 |
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