Crystallization (Comment) | Organism |
---|---|
homology modeling of structure | Thermochaetoides thermophila |
Protein Variants | Comment | Organism |
---|---|---|
Y173F | 1.9fold increased the enzyme's specific activity. Mutation significantly improves the enzymes heat resistance at 80°C and 90°C | Thermochaetoides thermophila |
Y30F | 1.4fold increased the enzyme's specific activity | Thermochaetoides thermophila |
Y30F/Y173F | mutant shows considerably higher stability at elevated temperatures but does not display the increased catalytic efficiency of its single mutant counterparts | Thermochaetoides thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermochaetoides thermophila | V9PD44 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.45 | - |
substrate beta-D-glucan, mutant Y30F/Y173F, pH 4.0, 60°C | Thermochaetoides thermophila |
0.68 | - |
substrate carboxymethylcellulose, mutant Y30F/Y173F, pH 4.0, 60°C | Thermochaetoides thermophila |
1 | - |
substrate beta-D-glucan, wild-type, pH 4.0, 60°C | Thermochaetoides thermophila |
1.21 | - |
substrate beta-D-glucan, mutant Y30F, pH 4.0, 60°C | Thermochaetoides thermophila |
1.21 | - |
substrate carboxymethylcellulose, wild-type, pH 4.0, 60°C | Thermochaetoides thermophila |
1.35 | - |
substrate carboxymethylcellulose, mutant Y30F, pH 4.0, 60°C | Thermochaetoides thermophila |
1.67 | - |
substrate beta-D-glucan, mutant Y173F, pH 4.0, 60°C | Thermochaetoides thermophila |
1.87 | - |
substrate carboxymethylcellulose, mutant Y173F, pH 4.0, 60°C | Thermochaetoides thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucan + H2O | - |
Thermochaetoides thermophila | ? | - |
? | |
carboxymethylcellulose + H2O | - |
Thermochaetoides thermophila | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
wild-type and mutants | Thermochaetoides thermophila |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
mutants Y30F and Y173F maintain more than 70% of maximum activity | Thermochaetoides thermophila |