Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | presence of Ca2+ increases the catalytic efficiency up to 40 % and remarkably raises the stability of the wild-type compared to the deletion mutant lacking the IgG-like domain | Alicyclobacillus acidocaldarius |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of the Ig-like domain in the N-terminus of the catalytic domain increases the catalytic efficiency of the truncated enzyme up to 3fold without any significant changes in the Km of the enzyme and shifts pH and temperature optimum for activity from 6.5 to 7.5 and from 65 to 60°C, respectively | Alicyclobacillus acidocaldarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
carboxymethylcellulose | Km of wild-type 7.75 mg/ml, of mutant lacking the IgG-like domain 7.24 mg/ml, respectively, pH 7.5, 65°C | Alicyclobacillus acidocaldarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alicyclobacillus acidocaldarius | Q9AJS0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethylcellulose + H2O | - |
Alicyclobacillus acidocaldarius | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 59000, wild.type, x * 49500, mutant lacking the IgG-like domain, SDS-PAGE and calculated | Alicyclobacillus acidocaldarius |
Synonyms | Comment | Organism |
---|---|---|
Cel9A | - |
Alicyclobacillus acidocaldarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
mutant lacking the IgG-like domain | Alicyclobacillus acidocaldarius |
65 | - |
wild-type | Alicyclobacillus acidocaldarius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
10 min, wild-type stable up to 30°C | Alicyclobacillus acidocaldarius |
60 | - |
10 min, mutant lacking the IgG-like domain stable up to 60°C | Alicyclobacillus acidocaldarius |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.75 | - |
carboxymethylcellulose | wild-type, pH 7.5, 65°C | Alicyclobacillus acidocaldarius | |
2.2 | - |
carboxymethylcellulose | mutant lacking the IgG-like domain, pH 7.5, 65°C | Alicyclobacillus acidocaldarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
wild-type | Alicyclobacillus acidocaldarius |
7.5 | - |
mutant lacking the IgG-like domain | Alicyclobacillus acidocaldarius |