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Literature summary for 3.2.1.4 extracted from

  • Younesi, F.; Pazhang, M.; Najavand, S.; Rahimizadeh, P.; Akbarian, M.; Mohammadian, M.; Khajeh, K.
    Deleting the Ig-like domain of Alicyclobacillus acidocaldarius endoglucanase Cel9A causes a simultaneous increase in the activity and stability (2016), Mol. Biotechnol., 58, 12-21 .
    View publication on PubMed
Search for more literature for 3.2.1.4

Activating Compound

Activating Compound Comment Organism Structure
Ca2+ presence of Ca2+ increases the catalytic efficiency up to 40 % and remarkably raises the stability of the wild-type compared to the deletion mutant lacking the IgG-like domain Alicyclobacillus acidocaldarius

Protein Variants

Protein Variants Comment Organism
additional information deletion of the Ig-like domain in the N-terminus of the catalytic domain increases the catalytic efficiency of the truncated enzyme up to 3fold without any significant changes in the Km of the enzyme and shifts pH and temperature optimum for activity from 6.5 to 7.5 and from 65 to 60°C, respectively Alicyclobacillus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 carboxymethylcellulose Km of wild-type 7.75 mg/ml, of mutant lacking the IgG-like domain 7.24 mg/ml, respectively, pH 7.5, 65°C Alicyclobacillus acidocaldarius

Organism

Organism UniProt Comment Textmining
Alicyclobacillus acidocaldarius Q9AJS0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylcellulose + H2O
-
 Alicyclobacillus acidocaldarius ?
-
 ?

Subunits

Subunits Comment Organism
? x * 59000, wild.type, x * 49500, mutant lacking the IgG-like domain, SDS-PAGE and calculated Alicyclobacillus acidocaldarius

Synonyms

Synonyms Comment Organism
Cel9A
-
 Alicyclobacillus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 mutant lacking the IgG-like domain Alicyclobacillus acidocaldarius
65
-
 wild-type Alicyclobacillus acidocaldarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 10 min, wild-type stable up to 30°C Alicyclobacillus acidocaldarius
60
-
 10 min, mutant lacking the IgG-like domain stable up to 60°C Alicyclobacillus acidocaldarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.75
-
 carboxymethylcellulose wild-type, pH 7.5, 65°C Alicyclobacillus acidocaldarius
2.2
-
 carboxymethylcellulose mutant lacking the IgG-like domain, pH 7.5, 65°C Alicyclobacillus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 wild-type Alicyclobacillus acidocaldarius
7.5
-
 mutant lacking the IgG-like domain Alicyclobacillus acidocaldarius