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Literature summary for 3.2.1.4 extracted from

  • Lee, J.; Seo, G.; An, S.; Kim, H.
    A cold-active acidophilic endoglucanase of Paenibacillus sp. Y2 isolated from soil in an alpine region (2017), J. Appl. Biol. Chem., 60, 257-263 .
No PubMed abstract available
Search for more literature for 3.2.1.4

Activating Compound

Activating Compound Comment Organism Structure
Cu2+ 5 mM, 136% of initial activity Paenibacillus sp. Y2
Mg2+ 5 mM, 127% of initial activity Paenibacillus sp. Y2
NaCl 0.5 M, 211% of initial activity Paenibacillus sp. Y2

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 5 mM, 43% of initial activity Paenibacillus sp. Y2
EDTA 5 mM, 63% of initial activity Paenibacillus sp. Y2
Fe2+ 5 mM, 77% of initial activity Paenibacillus sp. Y2
Mn2+ 5 mM, 60% of initial activity Paenibacillus sp. Y2
SDS 1%, 12% of initial activity Paenibacillus sp. Y2
Zn2+ 5 mM, 22% of initial activity Paenibacillus sp. Y2

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Paenibacillus sp. Y2
-
-

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. Y2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Paenibacillus sp. Y2

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.52
-
 pH 4.5, 30°C Paenibacillus sp. Y2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O
-
 Paenibacillus sp. Y2 ?
-
 ?
carboxymethylcellulose + H2O
-
 Paenibacillus sp. Y2 ?
-
 ?
lichenan + H2O
-
 Paenibacillus sp. Y2 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Paenibacillus sp. Y2

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Paenibacillus sp. Y2

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20
-
 79% of maximum activity Paenibacillus sp. Y2
50
-
 90% of maximum activity Paenibacillus sp. Y2

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-life 140 min Paenibacillus sp. Y2
55
-
 5 min, loss of activity Paenibacillus sp. Y2

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Paenibacillus sp. Y2

pH Range

pH Minimum pH Maximum Comment Organism
4 5.5 more than 60% of maximum activity Paenibacillus sp. Y2