Literature summary for 3.2.1.4 extracted from

Haq, I.U.; Akram, F.
Enhanced production of a recombinant multidomain thermostable GH9 processive endo-1,4-beta-glucanase (CenC) from Ruminiclostridium thermocellum in a mesophilic host through various cultivation and induction strategies (2017), Appl. Biochem. Biotechnol., 183, 171-188 .

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Application

Application	Comment	Organism
synthesis	enhanced production of enzyme in Escherichia coli. High-cell-density and optimal CenC expression are obtained in ZYBM9 medium induced either with 0.5 mM IPTG/150 mM lactose, after 6 h induction at 37°C. Before induction, bacterial cells are given heat shock (42°C) for 1 h when culture density (OD600 nm) reached at 0.6. Intracellular enzyme activity is enhanced by 6.67- and 3.20fold in ZYBM9 (yeast extract 0.5% (w/v), NaCl 0.5% (w/v), tryptone 1.0% (w/v), NH4Cl 0.1% (w/v), KH2PO4 0.3% (w/v), Na2HPO4 0.6% (w/v), MgSO4.7H2O 1 mM, and Glucose 0.4% (w/v)) and 3×ZYBM9 medium, respectively, under optimal conditions	Acetivibrio thermocellus

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Acetivibrio thermocellus

Organism

Organism	UniProt	Comment	Textmining
Acetivibrio thermocellus	-	-	-
Acetivibrio thermocellus ATCC 27405	-	-	-

Synonyms

Synonyms	Comment	Organism
CenC	-	Acetivibrio thermocellus

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Release 2023.1 (January 2023)