KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
carboxymethylcellulose | 1.67 mg/ml, pH 5.6, 50°C | Thermochaetoides thermophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
1 * 36000, SDS-PAGE | Thermochaetoides thermophila |
38000 | - |
gel filtration | Thermochaetoides thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermochaetoides thermophila | - |
var. coprophile | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Thermochaetoides thermophila |
Purification (Comment) | Organism |
---|---|
- |
Thermochaetoides thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethylcellulose + H2O | - |
Thermochaetoides thermophila | ? | - |
? | |
cellohexaose + H2O | the enzyme hydrolyzes cellotetraose, cellopentaose, and cellohexaose at comparable rates | Thermochaetoides thermophila | ? | - |
? | |
cellopentaose + H2O | the enzyme hydrolyzes cellotetraose, cellopentaose, and cellohexaose at comparable rates | Thermochaetoides thermophila | ? | - |
? | |
cellotetraose + H2O | the enzyme hydrolyzes cellotetraose, cellopentaose, and cellohexaose at comparable rates | Thermochaetoides thermophila | ? | - |
? | |
cellulose + H2O | - |
Thermochaetoides thermophila | cellobiose + cellotriose + cellotetraose + cellopentaose | - |
? | |
lichenan + H2O | - |
Thermochaetoides thermophila | ? | - |
? | |
additional information | the enzyme does not hydrolyze cellobiose and cellotriose | Thermochaetoides thermophila | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000, SDS-PAGE | Thermochaetoides thermophila |
Synonyms | Comment | Organism |
---|---|---|
(1->4)-beta-D-glucan 4-glucanohydrolase | - |
Thermochaetoides thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Thermochaetoides thermophila |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | 50°C: about 35% of maximal activity, 70°C: about 45% of maximal activity | Thermochaetoides thermophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable | Thermochaetoides thermophila |
60 | - |
t1/2: 18 min (without substrate), carboxymethyl cellulose partially protects the enzyme from heat inactivation | Thermochaetoides thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | 6.2 | - |
Thermochaetoides thermophila |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
64% of maximal activity | Thermochaetoides thermophila |