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Literature summary for 3.2.1.4 extracted from

  • Ganju, R. K.; S. K. Murthy; P. J., Vithayathil
    Purification and functional characteristics of an endocellulase from Chaetomium thermophile var. coprophile (1990), Carbohydr. Res., 197, 245-255.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
carboxymethylcellulose 1.67 mg/ml, pH 5.6, 50°C Thermochaetoides thermophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
1 * 36000, SDS-PAGE Thermochaetoides thermophila
38000
-
gel filtration Thermochaetoides thermophila

Organism

Organism UniProt Comment Textmining
Thermochaetoides thermophila
-
var. coprophile
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Thermochaetoides thermophila

Purification (Commentary)

Purification (Comment) Organism
-
Thermochaetoides thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylcellulose + H2O
-
Thermochaetoides thermophila ?
-
?
cellohexaose + H2O the enzyme hydrolyzes cellotetraose, cellopentaose, and cellohexaose at comparable rates Thermochaetoides thermophila ?
-
?
cellopentaose + H2O the enzyme hydrolyzes cellotetraose, cellopentaose, and cellohexaose at comparable rates Thermochaetoides thermophila ?
-
?
cellotetraose + H2O the enzyme hydrolyzes cellotetraose, cellopentaose, and cellohexaose at comparable rates Thermochaetoides thermophila ?
-
?
cellulose + H2O
-
Thermochaetoides thermophila cellobiose + cellotriose + cellotetraose + cellopentaose
-
?
lichenan + H2O
-
Thermochaetoides thermophila ?
-
?
additional information the enzyme does not hydrolyze cellobiose and cellotriose Thermochaetoides thermophila ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 36000, SDS-PAGE Thermochaetoides thermophila

Synonyms

Synonyms Comment Organism
(1->4)-beta-D-glucan 4-glucanohydrolase
-
Thermochaetoides thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Thermochaetoides thermophila

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 70 50°C: about 35% of maximal activity, 70°C: about 45% of maximal activity Thermochaetoides thermophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable Thermochaetoides thermophila
60
-
t1/2: 18 min (without substrate), carboxymethyl cellulose partially protects the enzyme from heat inactivation Thermochaetoides thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8 6.2
-
Thermochaetoides thermophila

pH Range

pH Minimum pH Maximum Comment Organism
8.8
-
64% of maximal activity Thermochaetoides thermophila