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Literature summary for 3.2.1.4 extracted from

  • Woo, M.H.; Chang, Y.H.; Lee, H.S.; Pak, P.J.; Kim, J.S.; Chung, N.
    First thermostable endo-beta-1,4-glucanase from newly isolated Xanthomonas sp. EC102 (2014), Protein J., 33, 110-117.
    View publication on PubMed
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Xanthomonas sp. EC102

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 carboxymethyl cellulose Km-value 25.6 mg/ml, pH 5.5, 70°C Xanthomonas sp. EC102

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 145% of initial activity Xanthomonas sp. EC102
Co2+ 149% of initial activity Xanthomonas sp. EC102

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 x * 52000, calculated Xanthomonas sp. EC102

Organism

Organism UniProt Comment Textmining
Xanthomonas sp. EC102 C7FFK1
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1970
-
 substrate carboxymethyl cellulose, pH 5.5, 30°C Xanthomonas sp. EC102

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellobioside + H2O 7.7% of the activity with carboxymethyl cellulose Xanthomonas sp. EC102 ?
-
 ?
avicel + H2O 55% of the activity with carboxymethyl cellulose Xanthomonas sp. EC102 ?
-
 ?
beechwood xylan + H2O 10% of the activity with carboxymethyl cellulose Xanthomonas sp. EC102 ?
-
 ?
carboxymethyl cellulose + H2O
-
 Xanthomonas sp. EC102 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 52000, calculated Xanthomonas sp. EC102

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Xanthomonas sp. EC102

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
80
-
 57% of maximum activity Xanthomonas sp. EC102

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Xanthomonas sp. EC102