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Literature summary for 3.2.1.4 extracted from
- Purification and characterization of an alkaline cellulase produced by Bacillus subtilis (AS3) (2013), Prep. Biochem. Biotechnol., 43, 256-270.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
carboxymethyl cellulose | Km value is 0.13 mg/ml, pH not specified in the publication, temperature not specified in the publication | Bacillus subtilis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus subtilis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
x * 30000, SDS-PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis AS3 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bagasse + H2O | steam-exploded bagasse | Bacillus subtilis | ? | - |
? |  |
| beta-glucan + H2O | and lichenan, preferred substrates | Bacillus subtilis | ? | - |
? | |
| beta-glucan + H2O | and lichenan, preferred substrates | Bacillus subtilis AS3 | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Bacillus subtilis | ? | - |
? | |
| carboxymethyl cellulose + H2O | - |
Bacillus subtilis AS3 | ? | - |
? | |
| hydroxyethyl cellulose + H2O | - |
Bacillus subtilis | ? | - |
? | |
| hydroxyethyl cellulose + H2O | - |
Bacillus subtilis AS3 | ? | - |
? | |
| laminarin + H2O | - |
Bacillus subtilis | ? | - |
? | |
| laminarin + H2O | - |
Bacillus subtilis AS3 | ? | - |
? | |
| lichenan + H2O | and beta-glucan, preferred substrates | Bacillus subtilis | ? | - |
? | |
| lichenan + H2O | and beta-glucan, preferred substrates | Bacillus subtilis AS3 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CMCase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | stable | Bacillus subtilis |
| 60 | - |
30 min , 50% residual activity | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.2 | - |
- |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | - |
Bacillus subtilis |
| 12 | - |
70% of maximum activity | Bacillus subtilis |
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Release 2023.1 (January 2023)
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