Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.4 extracted from

  • Bauer, M.W.; Driskill, L.E.; Callen, W.; Snead, M.A.; Mathur, E.J.; Kelly, R.M.
    An endoglucanase, EglA, from the hyperthermophilic archaeon Pyrococcus furiosus hydrolyzes beta-1,4 bonds in mixed-linkage (1-->3),(1-->4)-beta-D-glucans and cellulose (1999), J. Bacteriol., 181, 284-290.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
cellobiose
-
Pyrococcus furiosus
D-glucono-1,5-lactone
-
Pyrococcus furiosus
D-glucose
-
Pyrococcus furiosus
laminaribiose
-
Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.061
-
4-nitrophenyl cellotrioside pH 6.0, 95°C Pyrococcus furiosus
0.12
-
4-nitrophenyl cellotetraoside pH 6.0, 95°C Pyrococcus furiosus
0.15
-
4-nitrophenyl cellobioside pH 6.0, 95°C Pyrococcus furiosus
0.36
-
4-nitrophenyl cellopentaoside pH 6.0, 95°C Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35900
-
x * 35900, calculated from sequence Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q9V2T0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucoside + H2O low activity Pyrococcus furiosus 4-nitrophenol + beta-D-glucose
-
?
4-nitrophenyl cellobioside + H2O
-
Pyrococcus furiosus 4-nitrophenol + cellobiose
-
?
4-nitrophenyl cellopentaoside + H2O
-
Pyrococcus furiosus 4-nitrophenol + cellopentaose
-
?
4-nitrophenyl cellotetraoside + H2O
-
Pyrococcus furiosus 4-nitrophenol + cellotetraose
-
?
4-nitrophenyl cellotrioside + H2O
-
Pyrococcus furiosus 4-nitrophenol + cellotriose
-
?
barley beta-glucan + H2O the highest specific activity toward polysaccharides occurs with mixed-linkage (1->3),(1->4)-beta-D-glucans such as barley beta-glucan and lichenan Pyrococcus furiosus ?
-
?
cellohexaose + H2O
-
Pyrococcus furiosus ?
-
?
cellopentaose + H2O
-
Pyrococcus furiosus ?
-
?
lichenan + H2O the highest specific activity toward polysaccharides occurs with mixed-linkage (1->3),(1->4)-beta-D-glucans such as barley beta-D-glucan and lichenan Pyrococcus furiosus ?
-
?
additional information EglA hydrolyzes shorter cellodextrins (DP <5) as well as the amorphous portions of polysaccharides which contain only beta-1,4 bonds such as carboxymethyl cellulose, microcrystalline cellulose, Whatman paper, and cotton linter. Kinetics studies with cellooliogsaccharides and p-nitrophenyl-cellooligosaccharides indicated that the enzyme had three glucose binding subsites (-I, -II, and -III) for the nonreducing end and two glucose binding subsites (+I and +II) for the reducing end from the scissile glycosidic linkage. No activity is detected on solely beta-1,3-linked oligosaccharides or polysaccharides Pyrococcus furiosus ?
-
?

Subunits

Subunits Comment Organism
? x * 35900, calculated from sequence Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
EglA
-
Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
100
-
-
Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
half-life: 40 h Pyrococcus furiosus
112
-
denaturing temperature Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.79
-
4-nitrophenyl cellobioside pH 6.0, 95°C Pyrococcus furiosus
1.4
-
4-nitrophenyl cellopentaoside pH 6.0, 95°C Pyrococcus furiosus
3.6
-
4-nitrophenyl cellotetraoside pH 6.0, 95°C Pyrococcus furiosus
8.3
-
4-nitrophenyl cellotrioside pH 6.0, 95°C Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Pyrococcus furiosus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.1
-
D-glucono-1,5-lactone pH 6.0, 95°C Pyrococcus furiosus
1.6
-
laminaribiose pH 6.0, 95°C Pyrococcus furiosus
3
-
cellobiose pH 6.0, 95°C Pyrococcus furiosus
90
-
D-glucose pH 6.0, 95°C Pyrococcus furiosus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.06
-
4-nitrophenyl beta-D-glucoside pH 6.0, 95°C Pyrococcus furiosus
6.5
-
4-nitrophenyl cellopentaoside pH 6.0, 95°C Pyrococcus furiosus
8.7
-
4-nitrophenyl cellobioside pH 6.0, 95°C Pyrococcus furiosus
49
-
4-nitrophenyl cellotetraoside pH 6.0, 95°C Pyrococcus furiosus
220
-
4-nitrophenyl cellotrioside pH 6.0, 95°C Pyrococcus furiosus