BRENDA - Enzyme Database
show all sequences of 3.2.1.4

An endoglucanase, EglA, from the hyperthermophilic archaeon Pyrococcus furiosus hydrolyzes beta-1,4 bonds in mixed-linkage (1-->3),(1-->4)-beta-D-glucans and cellulose

Bauer, M.W.; Driskill, L.E.; Callen, W.; Snead, M.A.; Mathur, E.J.; Kelly, R.M.; J. Bacteriol. 181, 284-290 (1999)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli
Pyrococcus furiosus
Inhibitors
Inhibitors
Commentary
Organism
Structure
cellobiose
-
Pyrococcus furiosus
D-glucono-1,5-lactone
-
Pyrococcus furiosus
D-glucose
-
Pyrococcus furiosus
laminaribiose
-
Pyrococcus furiosus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.061
-
4-nitrophenyl cellotrioside
pH 6.0, 95°C
Pyrococcus furiosus
0.12
-
4-nitrophenyl cellotetraoside
pH 6.0, 95°C
Pyrococcus furiosus
0.15
-
4-nitrophenyl cellobioside
pH 6.0, 95°C
Pyrococcus furiosus
0.36
-
4-nitrophenyl cellopentaoside
pH 6.0, 95°C
Pyrococcus furiosus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35900
-
x * 35900, calculated from sequence
Pyrococcus furiosus
Organism
Organism
UniProt
Commentary
Textmining
Pyrococcus furiosus
Q9V2T0
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Pyrococcus furiosus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-nitrophenyl beta-D-glucoside + H2O
low activity
722499
Pyrococcus furiosus
4-nitrophenol + beta-D-glucose
-
-
-
?
4-nitrophenyl cellobioside + H2O
-
722499
Pyrococcus furiosus
4-nitrophenol + cellobiose
-
-
-
?
4-nitrophenyl cellopentaoside + H2O
-
722499
Pyrococcus furiosus
4-nitrophenol + cellopentaose
-
-
-
?
4-nitrophenyl cellotetraoside + H2O
-
722499
Pyrococcus furiosus
4-nitrophenol + cellotetraose
-
-
-
?
4-nitrophenyl cellotrioside + H2O
-
722499
Pyrococcus furiosus
4-nitrophenol + cellotriose
-
-
-
?
barley beta-glucan + H2O
the highest specific activity toward polysaccharides occurs with mixed-linkage (1->3),(1->4)-beta-D-glucans such as barley beta-glucan and lichenan
722499
Pyrococcus furiosus
?
-
-
-
?
cellohexaose + H2O
-
722499
Pyrococcus furiosus
?
-
-
-
?
cellopentaose + H2O
-
722499
Pyrococcus furiosus
?
-
-
-
?
lichenan + H2O
the highest specific activity toward polysaccharides occurs with mixed-linkage (1->3),(1->4)-beta-D-glucans such as barley beta-D-glucan and lichenan
722499
Pyrococcus furiosus
?
-
-
-
?
additional information
EglA hydrolyzes shorter cellodextrins (DP <5) as well as the amorphous portions of polysaccharides which contain only beta-1,4 bonds such as carboxymethyl cellulose, microcrystalline cellulose, Whatman paper, and cotton linter. Kinetics studies with cellooliogsaccharides and p-nitrophenyl-cellooligosaccharides indicated that the enzyme had three glucose binding subsites (-I, -II, and -III) for the nonreducing end and two glucose binding subsites (+I and +II) for the reducing end from the scissile glycosidic linkage. No activity is detected on solely beta-1,3-linked oligosaccharides or polysaccharides
722499
Pyrococcus furiosus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 35900, calculated from sequence
Pyrococcus furiosus
Synonyms
Synonyms
Commentary
Organism
EglA
-
Pyrococcus furiosus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
100
-
-
Pyrococcus furiosus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
95
-
half-life: 40 h
Pyrococcus furiosus
112
-
denaturing temperature
Pyrococcus furiosus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.79
-
4-nitrophenyl cellobioside
pH 6.0, 95°C
Pyrococcus furiosus
1.4
-
4-nitrophenyl cellopentaoside
pH 6.0, 95°C
Pyrococcus furiosus
3.6
-
4-nitrophenyl cellotetraoside
pH 6.0, 95°C
Pyrococcus furiosus
8.3
-
4-nitrophenyl cellotrioside
pH 6.0, 95°C
Pyrococcus furiosus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Pyrococcus furiosus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.1
-
D-glucono-1,5-lactone
pH 6.0, 95°C
Pyrococcus furiosus
1.6
-
laminaribiose
pH 6.0, 95°C
Pyrococcus furiosus
3
-
cellobiose
pH 6.0, 95°C
Pyrococcus furiosus
90
-
D-glucose
pH 6.0, 95°C
Pyrococcus furiosus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Pyrococcus furiosus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cellobiose
-
Pyrococcus furiosus
D-glucono-1,5-lactone
-
Pyrococcus furiosus
D-glucose
-
Pyrococcus furiosus
laminaribiose
-
Pyrococcus furiosus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.1
-
D-glucono-1,5-lactone
pH 6.0, 95°C
Pyrococcus furiosus
1.6
-
laminaribiose
pH 6.0, 95°C
Pyrococcus furiosus
3
-
cellobiose
pH 6.0, 95°C
Pyrococcus furiosus
90
-
D-glucose
pH 6.0, 95°C
Pyrococcus furiosus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.061
-
4-nitrophenyl cellotrioside
pH 6.0, 95°C
Pyrococcus furiosus
0.12
-
4-nitrophenyl cellotetraoside
pH 6.0, 95°C
Pyrococcus furiosus
0.15
-
4-nitrophenyl cellobioside
pH 6.0, 95°C
Pyrococcus furiosus
0.36
-
4-nitrophenyl cellopentaoside
pH 6.0, 95°C
Pyrococcus furiosus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35900
-
x * 35900, calculated from sequence
Pyrococcus furiosus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-nitrophenyl beta-D-glucoside + H2O
low activity
722499
Pyrococcus furiosus
4-nitrophenol + beta-D-glucose
-
-
-
?
4-nitrophenyl cellobioside + H2O
-
722499
Pyrococcus furiosus
4-nitrophenol + cellobiose
-
-
-
?
4-nitrophenyl cellopentaoside + H2O
-
722499
Pyrococcus furiosus
4-nitrophenol + cellopentaose
-
-
-
?
4-nitrophenyl cellotetraoside + H2O
-
722499
Pyrococcus furiosus
4-nitrophenol + cellotetraose
-
-
-
?
4-nitrophenyl cellotrioside + H2O
-
722499
Pyrococcus furiosus
4-nitrophenol + cellotriose
-
-
-
?
barley beta-glucan + H2O
the highest specific activity toward polysaccharides occurs with mixed-linkage (1->3),(1->4)-beta-D-glucans such as barley beta-glucan and lichenan
722499
Pyrococcus furiosus
?
-
-
-
?
cellohexaose + H2O
-
722499
Pyrococcus furiosus
?
-
-
-
?
cellopentaose + H2O
-
722499
Pyrococcus furiosus
?
-
-
-
?
lichenan + H2O
the highest specific activity toward polysaccharides occurs with mixed-linkage (1->3),(1->4)-beta-D-glucans such as barley beta-D-glucan and lichenan
722499
Pyrococcus furiosus
?
-
-
-
?
additional information
EglA hydrolyzes shorter cellodextrins (DP <5) as well as the amorphous portions of polysaccharides which contain only beta-1,4 bonds such as carboxymethyl cellulose, microcrystalline cellulose, Whatman paper, and cotton linter. Kinetics studies with cellooliogsaccharides and p-nitrophenyl-cellooligosaccharides indicated that the enzyme had three glucose binding subsites (-I, -II, and -III) for the nonreducing end and two glucose binding subsites (+I and +II) for the reducing end from the scissile glycosidic linkage. No activity is detected on solely beta-1,3-linked oligosaccharides or polysaccharides
722499
Pyrococcus furiosus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 35900, calculated from sequence
Pyrococcus furiosus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
100
-
-
Pyrococcus furiosus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
95
-
half-life: 40 h
Pyrococcus furiosus
112
-
denaturing temperature
Pyrococcus furiosus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.79
-
4-nitrophenyl cellobioside
pH 6.0, 95°C
Pyrococcus furiosus
1.4
-
4-nitrophenyl cellopentaoside
pH 6.0, 95°C
Pyrococcus furiosus
3.6
-
4-nitrophenyl cellotetraoside
pH 6.0, 95°C
Pyrococcus furiosus
8.3
-
4-nitrophenyl cellotrioside
pH 6.0, 95°C
Pyrococcus furiosus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Pyrococcus furiosus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.06
-
4-nitrophenyl beta-D-glucoside
pH 6.0, 95°C
Pyrococcus furiosus
6.5
-
4-nitrophenyl cellopentaoside
pH 6.0, 95°C
Pyrococcus furiosus
8.7
-
4-nitrophenyl cellobioside
pH 6.0, 95°C
Pyrococcus furiosus
49
-
4-nitrophenyl cellotetraoside
pH 6.0, 95°C
Pyrococcus furiosus
220
-
4-nitrophenyl cellotrioside
pH 6.0, 95°C
Pyrococcus furiosus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.06
-
4-nitrophenyl beta-D-glucoside
pH 6.0, 95°C
Pyrococcus furiosus
6.5
-
4-nitrophenyl cellopentaoside
pH 6.0, 95°C
Pyrococcus furiosus
8.7
-
4-nitrophenyl cellobioside
pH 6.0, 95°C
Pyrococcus furiosus
49
-
4-nitrophenyl cellotetraoside
pH 6.0, 95°C
Pyrococcus furiosus
220
-
4-nitrophenyl cellotrioside
pH 6.0, 95°C
Pyrococcus furiosus
Other publictions for EC 3.2.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)