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Literature summary for 3.2.1.4 extracted from
- Cloning and expression of a novel thermostable cellulase from newly isolated Bacillus subtilis strain I15 (2010), Mol. Biol. Rep., 37, 1923-1929.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene celI15, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain BL21(DE3) as extracellular enzyme, subcloning in Escherichia coli strain JM109 | Bacillus subtilis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus subtilis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
x * 52000, recombinant extracellular enzyme, SDS-PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | B7UAM4 | isolated from the longtime-thermal compost containing rich cellulose materials in a factory of Zhengzhou in China, gene celI15 | - |
| Bacillus subtilis I15 | B7UAM4 | isolated from the longtime-thermal compost containing rich cellulose materials in a factory of Zhengzhou in China, gene celI15 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
2.82 U/ml for the native extracellular enzyme, 6.78-8.54 U/ml for the recombinant extracellular enzyme expressed in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethyl-cellulose + H2O | - |
Bacillus subtilis | ? | - |
? |  |
| carboxymethyl-cellulose + H2O | - |
Bacillus subtilis I15 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52000, recombinant extracellular enzyme, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CelI15 | - |
Bacillus subtilis |
| CMCase | - |
Bacillus subtilis |
| endo-beta-1,4-glucanase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 65 | - |
Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 95 | activity range, profile, overview | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
2 h, extracellular enzyme, 90% activity remaining | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | activity range, profile, overview | Bacillus subtilis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | cellulolytic enzymes, including endo-beta-1,4-glucanases, EC 3.2.1.4, exo-beta-1,4-glucanases, EC 3.2.1.91, and beta-glucosidases, EC 3.2.1.21, collectively known as cellulases, act in a synergistic manner to facilitate complete cleavage of the cellulose beta-1,4-glycosidic bonds to form glucose | Bacillus subtilis |
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