Literature summary for 3.2.1.4 extracted from
Wang, C.; Hsieh, Y.; Ng, C.; Chan, H.; Lin, H.; Tzeng, W.; Shyu, Y.
Purification and characterization of a novel halostable cellulase from Salinivibrio sp. strain NTU-05 (2009), Enzyme Microb. Technol., 44, 373-379.
No PubMed abstract available
KM Value [mM]
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
additional information |
- |
additional information |
kinetics, native enzyme |
Salinivibrio sp. NTU-05 |
|
Localization
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
cellulosome |
- |
Salinivibrio sp. NTU-05 |
43263 |
- |
Metals/Ions
Metals/Ions |
Comment |
Organism |
Structure |
Ca2+ |
activates |
Salinivibrio sp. NTU-05 |
|
Cu2+ |
activates |
Salinivibrio sp. NTU-05 |
|
Fe3+ |
activates |
Salinivibrio sp. NTU-05 |
|
Hg2+ |
activates |
Salinivibrio sp. NTU-05 |
|
K+ |
activates |
Salinivibrio sp. NTU-05 |
|
Mg2+ |
activates |
Salinivibrio sp. NTU-05 |
|
Mn2+ |
activates |
Salinivibrio sp. NTU-05 |
|
Na+ |
activate sat 0-20% v/w, optimally at 5% v/w |
Salinivibrio sp. NTU-05 |
|
Zn2+ |
activates |
Salinivibrio sp. NTU-05 |
|
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
29000 |
- |
gel filtration |
Salinivibrio sp. NTU-05 |
Organism
Organism |
UniProt |
Comment |
Textmining |
Salinivibrio sp. NTU-05 |
- |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
native enzyme 29.5fold by anion exchange chromatography and gel filtration |
Salinivibrio sp. NTU-05 |
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
32.4 |
- |
purified native enzyme |
Salinivibrio sp. NTU-05 |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
additional information |
carboxymethyl cellulose and beta-glucan are the best substrates with relative activities of 100% and 83%, respectively, birch wood xylan and oat spelt xylan have moderate activities of 68% and 60%, respectively, which otherwise supports low level of cellulase activity, Avicel has the lowest activity of 12%, substrate specificity, overview |
Salinivibrio sp. NTU-05 |
? |
- |
? |
|
Subunits
Subunits |
Comment |
Organism |
monomer |
1 * 29000, SDS-PAGE |
Salinivibrio sp. NTU-05 |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
35 |
- |
- |
Salinivibrio sp. NTU-05 |
Temperature Range [°C]
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
10 |
60 |
activity range, profile, overview |
Salinivibrio sp. NTU-05 |
Temperature Stability [°C]
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
10 |
70 |
purified native enzyme, highly stable in the temperature range of 10-40°C, the activity declines rapidly as the temperature increases above 60°C, 12% activity remains at 70°C after 4 h. At 10°C, the enzyme exhibits no loss of activity, even after 24 h |
Salinivibrio sp. NTU-05 |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
7.5 |
- |
- |
Salinivibrio sp. NTU-05 |
pH Range
pH Minimum |
pH Maximum |
Comment |
Organism |
5 |
9 |
activity range, 80% of maximal activity at pH 6.5 and pH 8.5, profile, overview |
Salinivibrio sp. NTU-05 |
pH Stability
pH Stability |
pH Stability Maximum |
Comment |
Organism |
5 |
8 |
the purified native enzyme retains 100% of its activity at pH 7.0 and pH 8.0 for 24 h, but only 35% at pH 5.0 |
Salinivibrio sp. NTU-05 |