Cloned (Comment) | Organism |
---|---|
gene bgl7A, overexpression in Pichia pastoris strain GS115 | Bispora sp. MEY-1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Bispora sp. MEY-1 | |
Co2+ | - |
Bispora sp. MEY-1 | |
Cr3+ | - |
Bispora sp. MEY-1 | |
Cu2+ | - |
Bispora sp. MEY-1 | |
EDTA | - |
Bispora sp. MEY-1 | |
Fe3+ | - |
Bispora sp. MEY-1 | |
Hg2+ | - |
Bispora sp. MEY-1 | |
Mg2+ | - |
Bispora sp. MEY-1 | |
Mn2+ | - |
Bispora sp. MEY-1 | |
additional information | BglA7 is highly resistant to both pepsin and trypsin | Bispora sp. MEY-1 | |
Ni2+ | - |
Bispora sp. MEY-1 | |
Pb2+ | - |
Bispora sp. MEY-1 | |
SDS | - |
Bispora sp. MEY-1 | |
Zn2+ | - |
Bispora sp. MEY-1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzymatic activity is enhanced or not affected by the presence of Na+, K+, Li+, Ca2+, or 2-mercaptoethanol | Bispora sp. MEY-1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
x * 48000, deglycosylated Bgl7A, SDS-PAGE, x * 60000-80000, recombinant glycosylated enzyme, SDS-PAGE | Bispora sp. MEY-1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bispora sp. MEY-1 | Bgl7A can effectively hydrolyze beta-1,4 bonds and some beta-1,3 linkages in beta-glucan. Belonging to the group of non-specific endoglucanase, Bgl7A can hydrolyze not only beta-glucan and cellulose but also laminarin and oat spelt xylan | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bispora sp. MEY-1 | D2DRB6 | acidophilic fungus, CGMCC 2500, gene bgl7A | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Bispora sp. MEY-1 |
Purification (Comment) | Organism |
---|---|
recombinant Bgl7A 1.3fold from Pichia pastoris strain GS115 by ion exchange chromatography | Bispora sp. MEY-1 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2740 | - |
substrate lichenan | Bispora sp. MEY-1 |
4040 | - |
substrate Hordeum vulgare beta-glucan | Bispora sp. MEY-1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-glucan + H2O | from Hordeum vulgare | Bispora sp. MEY-1 | ? | - |
? | |
carboxymethylcellulose + H2O | - |
Bispora sp. MEY-1 | ? | - |
? | |
lichenan + H2O | - |
Bispora sp. MEY-1 | ? | - |
? | |
additional information | Bgl7A can effectively hydrolyze beta-1,4 bonds and some beta-1,3 linkages in beta-glucan. Belonging to the group of non-specific endoglucanase, Bgl7A can hydrolyze not only beta-glucan and cellulose but also laminarin and oat spelt xylan | Bispora sp. MEY-1 | ? | - |
? | |
additional information | poor activity with laminarin and oat spelt xylan, no activity with Avicel | Bispora sp. MEY-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, deglycosylated Bgl7A, SDS-PAGE, x * 60000-80000, recombinant glycosylated enzyme, SDS-PAGE | Bispora sp. MEY-1 |
Synonyms | Comment | Organism |
---|---|---|
Bgl7A | - |
Bispora sp. MEY-1 |
endo-beta-1,3-1,4-glucanase | - |
Bispora sp. MEY-1 |
More | the enzyme belongs to the acidic glycoside hydrolase family 7, GH7 | Bispora sp. MEY-1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bispora sp. MEY-1 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 80 | activity rpofile, overview | Bispora sp. MEY-1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified recombinant enzyme, 60 min, stable | Bispora sp. MEY-1 |
70 | - |
purified enzyme, 48% activity remaining after 5 min, 30% after 60 min | Bispora sp. MEY-1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant Bgl7A has three activity peaks at pH 1.5, pH 3.5, and pH 5.0, the last is the highest | Bispora sp. MEY-1 |
5 | - |
- |
Bispora sp. MEY-1 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
1 | 6 | activity rpofile, overview | Bispora sp. MEY-1 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | 8 | purified recombinant enzyme, stable, over 85% within this range | Bispora sp. MEY-1 |