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Literature summary for 3.2.1.4 extracted from

  • Shanmughapriya, S.; Kiran, G.S.; Selvin, J.; Thomas, T.A.; Rani, C.
    Optimization, purification, and characterization of extracellular mesophilic alkaline cellulase from sponge-associated Marinobacter sp. MSI032 (2009), Appl. Biochem. Biotechnol., 162, 625-640.
    View publication on PubMed
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic tree and analysis Marinobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Marinobacter sp.
Fe2+
-
 Marinobacter sp.
Mn2+
-
 Marinobacter sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Marinobacter sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ slightly activating Marinobacter sp.
additional information enzyme activity is not affected by Hg2+ Marinobacter sp.
NaCl 100% activation at 2.5 M Marinobacter sp.
Zn2+ highly activating Marinobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 x * 68000, SDS-PAGE Marinobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellulose + H2O Marinobacter sp.
-
 ?
-
 ?
cellulose + H2O Marinobacter sp. MSI032
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Marinobacter sp.
-
 isolated from the marine sponge Dendrilla nigra
-
Marinobacter sp. MSI032
-
 isolated from the marine sponge Dendrilla nigra
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 12.5fold from culture medium by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography Marinobacter sp.

Source Tissue

Source Tissue Comment Organism Textmining
additional information the culture medium containing 1% maltose as carbon source and 1% peptone and casein as nitrogen source, and with presence of 2% NaCl and 10 mM Zn2+, supports maximal enzyme production at 27°C and at a pH of 9.0 Marinobacter sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2549
-
 purified native enzyme Marinobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellulose + H2O
-
 Marinobacter sp. ?
-
 ?
cellulose + H2O
-
 Marinobacter sp. MSI032 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 68000, SDS-PAGE Marinobacter sp.

Synonyms

Synonyms Comment Organism
Alkaline cellulase
-
 Marinobacter sp.
CMCase
-
 Marinobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27 35
-
 Marinobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
27 30 purified enzyme, 60 min, completely stable Marinobacter sp.
37
-
 purified enzyme, loss of 60% activity after 60 min Marinobacter sp.
40
-
 purified enzyme, inactivation after 60 min Marinobacter sp.
50
-
 purified enzyme, inactivation after 40 min Marinobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Marinobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 12 the enzyme shows a broad pH range with 50% of maximal activity at pH 7.0 and maximal activity at pH 9.0 Marinobacter sp.