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Literature summary for 3.2.1.4 extracted from

  • Koga, J.; Baba, Y.; Shimonaka, A.; Nishimura, T.; Hanamura, S.; Kono, T.
    Purification and characterization of a new family 45 endoglucanase, STCE1, from Staphylotrichum coccosporum and its overproduction in Humicola insolens (2008), Appl. Environ. Microbiol., 74, 4210-4217.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.4

Application

Application Comment Organism
industry STCE1 may be appropriate for laundry use Staphylotrichum coccosporum

Cloned(Commentary)

Cloned (Comment) Organism
overproduction in Humicola insolens Staphylotrichum coccosporum

Inhibitors

Inhibitors Comment Organism Structure
linear alkyl benzene sulfonate LAS Staphylotrichum coccosporum
sodium hypochlorite
-
 Staphylotrichum coccosporum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32650
-
 calculated molecular weight of mature STCE1 Staphylotrichum coccosporum
49000
-
 SDS-PAGE Staphylotrichum coccosporum

Organism

Organism UniProt Comment Textmining
Staphylotrichum coccosporum B5BNY1 NBRC 31817
-
Staphylotrichum coccosporum NBRC 31817 B5BNY1 NBRC 31817
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Staphylotrichum coccosporum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
 Staphylotrichum coccosporum ?
-
 ?
carboxymethyl cellulose + H2O
-
 Staphylotrichum coccosporum NBRC 31817 ?
-
 ?

Subunits

Subunits Comment Organism
More STCE1 consists of three distinct domains: an N-terminal catalytic domain (family 45), a linker domain, and a C-terminal carbohydrate-binding module (family 1) Staphylotrichum coccosporum

Synonyms

Synonyms Comment Organism
Carboxymethyl cellulase
-
 Staphylotrichum coccosporum
CMCase
-
 Staphylotrichum coccosporum
endoglucanase
-
 Staphylotrichum coccosporum
STCE1
-
 Staphylotrichum coccosporum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Staphylotrichum coccosporum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 for the carboxymethyl cellulase activity Staphylotrichum coccosporum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
 0.303 mM for linear alkyl benzene sulfonate Staphylotrichum coccosporum additional information
0.039
-
-
 Staphylotrichum coccosporum sodium hypochlorite