Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-chymotrypsin | denaturant | Aspergillus oryzae | |
Urea | denaturant | Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | KM value: 20 mg carboxymethylcellulose/ml. Vmax value: 854 U/mg protein, one unit defined as the release of 1 mircomol of glucose equivalent per minute from carboxymethylcellulose | Aspergillus oryzae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus oryzae | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
SDS-PAGE | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
cmc-1 | - |
Aspergillus oryzae cmc-1 | - |
cmc-1 | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and various column chromatography on fast protein liquid chromatography system to homogeneity | Aspergillus oryzae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
92.5 | - |
gel filtration chromatography, last purification step | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethylcellulose + H2O | - |
Aspergillus oryzae | ? | - |
? | |
carboxymethylcellulose + H2O | - |
Aspergillus oryzae cmc-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | SDS-PAGE | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
CMCase | - |
Aspergillus oryzae |
endoglucanase | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | - |
enzyme is stable up to 47°C and exhibits half-life of 138.6 min | Aspergillus oryzae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 356 1/s, one unit defined as the release of 1 mircomol of glucose equivalent per minute from carboxymethylcellulose | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.4 | - |
- |
Aspergillus oryzae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.1 | 5.3 | enzyme is stable over a pH range of 4.1-5.3 | Aspergillus oryzae |