Literature summary for 3.2.1.4 extracted from

Yasutake, Y.; Kawano, S.; Tajima, K.; Yao, M.; Satoh, Y.; Munekata, M.; Tanaka, I.
Structural characterization of the Acetobacter xylinum endo-beta-1,4-glucanase CMCax required for cellulose biosynthesis (2006), Proteins, 64, 1069-1077.

Cloned(Commentary)

Cloned (Comment)	Organism
selenomethionine-substituted CMCax is overexpressed in Escherichia coli B834 Codon Plus (DE3)-IRL (Stratagene) with a (His)6-tag at the N-terminus in place of the signal peptide	Komagataeibacter xylinus

Crystallization (Commentary)

Crystallization (Comment)	Organism
a single crystal of CMCax (Crystal I) is grown at 20°C by the hanging drop vapor diffusion method against a reservoir solution containing 100 mM sodium citrate buffer, pH 4.4, 15% (w/v) PEG 4,000, 3% (w/v) PEG 8,000, and 20 mM ammonium sulfate. Crystal belongs to the space group P6(1) with unit cell dimensions of a = b = 89.1 A and c = 94.2 A. Another crystal of larger size (Crystal II) is obtained under the above conditions but with the addition of 10 mM cellobiose. Prior to X-ray diffraction experiments, the crystal is rapidly soaked in reservoir solution supplemented with 20% (v/v) glycerol, and flash-cooled under a stream of nitrogen gas. The crystal diffracted to a resolution of 1.65 A	Komagataeibacter xylinus

Crystallization (Comment)

Organism

a single crystal of CMCax (Crystal I) is grown at 20°C by the hanging drop vapor diffusion method against a reservoir solution containing 100 mM sodium citrate buffer, pH 4.4, 15% (w/v) PEG 4,000, 3% (w/v) PEG 8,000, and 20 mM ammonium sulfate. Crystal belongs to the space group P6(1) with unit cell dimensions of a = b = 89.1 A and c = 94.2 A. Another crystal of larger size (Crystal II) is obtained under the above conditions but with the addition of 10 mM cellobiose. Prior to X-ray diffraction experiments, the crystal is rapidly soaked in reservoir solution supplemented with 20% (v/v) glycerol, and flash-cooled under a stream of nitrogen gas. The crystal diffracted to a resolution of 1.65 A

Komagataeibacter xylinus

Organism

Organism	UniProt	Comment	Textmining
Komagataeibacter xylinus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Komagataeibacter xylinus

Synonyms

Synonyms	Comment	Organism
endo-beta-1,4-glucanase CMCax	-	Komagataeibacter xylinus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)