Protein Variants | Comment | Organism |
---|---|---|
D385N | activity towards carboxymethyl cellulose is 29.9% of wild-type activity | Pyrococcus horikoshii |
DELTAQ1-G5 | activity towards carboxymethyl cellulose is 135.6% of wild-type activity. kcat/Km for p-nitrophenyl cellobiose is 2.3fold higher than wild-type value. Thermostability is not significantly influenced | Pyrococcus horikoshii |
E201A | activity towards carboxymethyl cellulose is 0.01% of wild-type activity | Pyrococcus horikoshii |
E201Q | activity towards carboxymethyl cellulose is 1.12% of wild-type activity. kcat/Km for p-nitrophenyl cellobiose is 43fold lower than wild-type value | Pyrococcus horikoshii |
E342A | activity towards carboxymethyl cellulose is 0.08% of wild-type activity, no activity with p-nitrophenyl cellobiose | Pyrococcus horikoshii |
E342Q | activity towards carboxymethyl cellulose is 0.01% of wild-type activity | Pyrococcus horikoshii |
H297A | activity towards carboxymethyl cellulose is 0.08% of wild-type activity | Pyrococcus horikoshii |
H297N | activity towards carboxymethyl cellulose is 1.31% of wild-type activity. pH-optimum is 7.0, compared to 5.5-6 for wild-type enzyme | Pyrococcus horikoshii |
additional information | mutant enzyme lacking 5 residues at the C-terminus: hydrolytic activity towards carboxymethyl cellulose is 112% of wild-type activity, kcat/Km for p-nitrophenyl cellobiose is 1.2fold higher than wild-type value. Mutant enzyme lacking 5 residues at the C-terminus and 5 residues at the N-terminus: activity towards carboxymethyl cellulose is 111% of wild-type activity, kcat/Km for p-nitrophenyl cellobiose is 1.8fold higher than wild-type value. Thermostability is not significantly influenced | Pyrococcus horikoshii |
N200A | activity towards carboxymethyl cellulose is 5.43% of wild-type activity | Pyrococcus horikoshii |
R102A | activity towards carboxymethyl cellulose is 0.67% of wild-type activity | Pyrococcus horikoshii |
W377A | activity towards carboxymethyl cellulose is 1.02% of wild-type activity | Pyrococcus horikoshii |
Y299A | activity towards carboxymethyl cellulose is 0.21% of wild-type activity | Pyrococcus horikoshii |
Y299F | activity towards carboxymethyl cellulose is 2.15% of wild-type activity. pH-optimum is 8.5, compared to 5.5-6 for wild-type enzyme | Pyrococcus horikoshii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.76 | - |
p-nitrophenyl cellobiose | pH 5-6, 50°C, mutant enzyme DELTAQ1-G5 | Pyrococcus horikoshii | |
0.95 | - |
p-nitrophenyl cellobiose | pH 5-6, 50°C, wild-type enzyme | Pyrococcus horikoshii | |
1.12 | - |
p-nitrophenyl cellobiose | pH 5-6, 50°C, mutant enzyme Y299F | Pyrococcus horikoshii | |
1.13 | - |
p-nitrophenyl cellobiose | pH 5-6, 50°C, mutant enzyme lacking 5 residues at the C-terminus and 5 residies at the N-terminus | Pyrococcus horikoshii | |
1.24 | - |
p-nitrophenyl cellobiose | pH 5-6, 50°C, mutant enzyme E201Q | Pyrococcus horikoshii | |
1.44 | - |
p-nitrophenyl cellobiose | pH 5-6, 50°C, mutant enzyme lacking 5 residues at the C-terminus | Pyrococcus horikoshii | |
2.4 | - |
p-nitrophenyl cellobiose | pH 5-6, 50°C, mutant enzyme D385N | Pyrococcus horikoshii | |
2.76 | - |
p-nitrophenyl cellobiose | pH 5-6, 50°C, mutant enzyme H297N | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O58925 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl cellulose + H2O | - |
Pyrococcus horikoshii | ? | - |
? | |
p-nitrophenyl cellobiose + H2O | - |
Pyrococcus horikoshii | p-nitrophenol + cellobiose | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
p-nitrophenyl cellobiose | pH 5-6, 50°C, mutant enzyme E201Q | Pyrococcus horikoshii | |
0.01 | - |
p-nitrophenyl cellobiose | pH 5-6, 50°C, mutant enzyme H297N | Pyrococcus horikoshii | |
0.01 | - |
p-nitrophenyl cellobiose | pH 5-6, 50°C, mutant enzyme Y299F | Pyrococcus horikoshii | |
0.11 | - |
p-nitrophenyl cellobiose | pH 5-6, 50°C, mutant enzyme D385N | Pyrococcus horikoshii | |
0.41 | - |
p-nitrophenyl cellobiose | pH 5-6, 50°C, wild-type enzyme | Pyrococcus horikoshii | |
0.75 | - |
p-nitrophenyl cellobiose | pH 5-6, 50°C, mutant enzyme lacking 5 residues at the C-terminus | Pyrococcus horikoshii | |
0.78 | - |
p-nitrophenyl cellobiose | pH 5-6, 50°C, mutant enzyme DELTAQ1-G5 | Pyrococcus horikoshii | |
0.91 | - |
p-nitrophenyl cellobiose | pH 5-6, 50°C, mutant enzyme lacking 5 residues at the C-terminus and 5 residies at the N-terminus | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | optimal pH for wild-type enzyme and mutant enzymes E201Q, E342Q, and D385N | Pyrococcus horikoshii |
7 | - |
mutant enzyme H297N | Pyrococcus horikoshii |
8.5 | - |
mutant enzyme Y299F | Pyrococcus horikoshii |