Cloned (Comment) | Organism |
---|---|
- |
Bacillus amyloliquefaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoCl2 | 5 mM, 35% loss of activity | Bacillus amyloliquefaciens | |
EDTA | 5 mM, 75% loss of activity | Bacillus amyloliquefaciens | |
HgCl2 | 5 mM, 76% loss of activity | Bacillus amyloliquefaciens | |
KCl | 5 mM, 23% loss of activity | Bacillus amyloliquefaciens | |
MnCl2 | 5 mM, 67% loss of activity | Bacillus amyloliquefaciens | |
N-bromosuccinimide | 5 mM, 56% loss of activity | Bacillus amyloliquefaciens | |
NiCl2 | 5 mM, 40% loss of activity | Bacillus amyloliquefaciens | |
PbCl2 | 5 mM, 37% loss of activity | Bacillus amyloliquefaciens | |
SrCl2 | 5 mM, 36% loss of activity | Bacillus amyloliquefaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 5 mM, activation to 109% of control | Bacillus amyloliquefaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
x * 54000, SDS-PAGE | Bacillus amyloliquefaciens |
55118 | - |
x * 55118, calculated from sequence | Bacillus amyloliquefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
- |
- |
Bacillus amyloliquefaciens DL-3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus amyloliquefaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
60 | 70 | - |
Bacillus amyloliquefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
avicel + H2O | 14% of the activity with carboxymethylcellulose | Bacillus amyloliquefaciens | ? | - |
? | |
avicel + H2O | 14% of the activity with carboxymethylcellulose | Bacillus amyloliquefaciens DL-3 | ? | - |
? | |
beta-glucan + H2O | 24.6% of the activity with carboxymethylcellulose | Bacillus amyloliquefaciens | ? | - |
? | |
beta-glucan + H2O | 24.6% of the activity with carboxymethylcellulose | Bacillus amyloliquefaciens DL-3 | ? | - |
? | |
carboxymethylcellulose + H2O | - |
Bacillus amyloliquefaciens | ? | - |
? | |
carboxymethylcellulose + H2O | - |
Bacillus amyloliquefaciens DL-3 | ? | - |
? | |
cellobiose + H2O | 8.6% of the activity with carboxymethylcellulose | Bacillus amyloliquefaciens | ? | - |
? | |
additional information | no activity with p-nitrophenyl-beta-D-glucopyranoside | Bacillus amyloliquefaciens | ? | - |
? | |
additional information | no activity with p-nitrophenyl-beta-D-glucopyranoside | Bacillus amyloliquefaciens DL-3 | ? | - |
? | |
xylan + H2O | 22.5% of the activity with carboxymethylcellulose | Bacillus amyloliquefaciens | ? | - |
? | |
xylan + H2O | 22.5% of the activity with carboxymethylcellulose | Bacillus amyloliquefaciens DL-3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, SDS-PAGE | Bacillus amyloliquefaciens |
? | x * 55118, calculated from sequence | Bacillus amyloliquefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 8.0, substrate: carboxymethylcellulose | Bacillus amyloliquefaciens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | 20°C: 65.9% of maximal activity, 80°C: 72.3% of maximal activity, substrate: carboxymethylcellulose | Bacillus amyloliquefaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 30 | pH 8.0, 20 h, about 60%% loss of activity | Bacillus amyloliquefaciens |
40 | - |
pH 8.0, 20 h, about 40% loss of activity | Bacillus amyloliquefaciens |
50 | - |
pH 8.0, 20 h, about 10% loss of activity | Bacillus amyloliquefaciens |
60 | - |
pH 8.0, 20 h, about 15% loss of activity | Bacillus amyloliquefaciens |
70 | - |
pH 8.0, 20 h, about 20% loss of activity | Bacillus amyloliquefaciens |
80 | - |
pH 8.0, 20 h, about 40% loss of activity | Bacillus amyloliquefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
substrate: carboxymethylcellulose | Bacillus amyloliquefaciens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 11 | pH 3: about 55% of maximal activity, pH 11: about 45% of maximal activity | Bacillus amyloliquefaciens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | 50°C, 20 h, stable | Bacillus amyloliquefaciens |