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Literature summary for 3.2.1.4 extracted from

  • Kim, J.Y.; Hur, S.H.; Hong, J.H.
    Purification and characterization of an alkaline cellulase from a newly isolated alkalophilic Bacillus sp. HSH-810 (2005), Biotechnol. Lett., 27, 313-316.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Fe3+ 39% inhibition by 0.1 mM, 52% inhibition by 1 mM Bacillus sp. (in: Bacteria)
Hg2+ 21% inhibition at 1 mM Bacillus sp. (in: Bacteria)
Mg2+ 31% inhibition at 1 mM Bacillus sp. (in: Bacteria)
Mn2+ 54% inhibition at 0.1 mM, 71% inhibition at 1 mM Bacillus sp. (in: Bacteria)
Pb(CH3COO)2 34% inhibition at 1 mM Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
x * 80000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) HSH-810
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
71
-
-
Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
Bacillus sp. (in: Bacteria) ?
-
?
carboxymethyl cellulose + H2O
-
Bacillus sp. (in: Bacteria) HSH-810 ?
-
?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
60 min, about 20% loss of activity Bacillus sp. (in: Bacteria)
60
-
60 min, more than 60% of the initial activity remains Bacillus sp. (in: Bacteria)
70
-
60 min, about 60% loss of activity Bacillus sp. (in: Bacteria)
80
-
40 min, about 80% loss of activity Bacillus sp. (in: Bacteria)
90
-
20 min, about 90% loss of acfivity Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
8.5 12.5 pH 8.5: about 45% of maximal activity, pH 12.5: about 45% of maximal activity Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
1 2 5°C, 24 h, about 30% loss of activity Bacillus sp. (in: Bacteria)
1 3 5°C, 24 h, about 80% loss of activity Bacillus sp. (in: Bacteria)
4
-
5°C, 24 h, about 40% loss of activity Bacillus sp. (in: Bacteria)
5
-
5°C, 24 h, about 30% loss of activity Bacillus sp. (in: Bacteria)
6
-
5°C, 24 h, about 15% loss of activity Bacillus sp. (in: Bacteria)
7
-
5°C, 24 h, about 5% loss of activity Bacillus sp. (in: Bacteria)
8 9 5°C, 24 h, stable Bacillus sp. (in: Bacteria)
10
-
5°C, 24 h, about 15% loss of activity Bacillus sp. (in: Bacteria)
11
-
5°C, 24 h, about 25% loss of activity Bacillus sp. (in: Bacteria)