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Literature summary for 3.2.1.4 extracted from
- Purification and characterization of a cellulase from the ruminal fungus Orpinomyces joyonii cloned in Escherichia coli (2001), Int. J. Biochem. Cell Biol., 33, 87-94.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Orpinomyces joyonii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the Km-values for barley beta-glucan and carboxymethylcellulose are 0.32 mg/ml and 0.5 mg/ml | Orpinomyces joyonii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56463 | - |
x * 56463, calculation from nucleotide sequence | Orpinomyces joyonii |
| 58000 | - |
x * 58000, SDS-PAGE | Orpinomyces joyonii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Orpinomyces joyonii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Orpinomyces joyonii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-glucan + H2O | - |
Orpinomyces joyonii | ? | - |
? |  |
| carboxymethyl cellulose + H2O | 68% of the activity with barley beta-glucan | Orpinomyces joyonii | ? | - |
? | |
| cellobioside + H2O | about 2% of the activity with barley beta-glucan | Orpinomyces joyonii | ? | - |
? | |
| cellotrioside + H2O | 7.4% of the activity with barley beta-glucan | Orpinomyces joyonii | ? | - |
? | |
| lichenan + H2O | 9% of the activity with barley beta-glucan | Orpinomyces joyonii | ? | - |
? | |
| additional information | no activity with laminarin, avicel, pullulan and pachyman | Orpinomyces joyonii | ? | - |
? | |
| xylan + H2O | about 2% of the activity with barley beta-glucan | Orpinomyces joyonii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 58000, SDS-PAGE | Orpinomyces joyonii |
| ? | x * 56463, calculation from nucleotide sequence | Orpinomyces joyonii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
reaction with carboxymethyl cellulose or barley beta-glucan | Orpinomyces joyonii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
10 min, stable | Orpinomyces joyonii |
| 50 | - |
10 min, about 10% loss of activity | Orpinomyces joyonii |
| 55 | - |
10 min, about 30% loss of activity with barley beta-glucan and about 60% loss of activity with carboxymethylcellulose | Orpinomyces joyonii |
| 60 | - |
10 min, about 50% loss of activity with barley beta-glucan and about 75% loss of activity with carboxymethylcellulose | Orpinomyces joyonii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
reaction with carboxymethyl cellulose | Orpinomyces joyonii |
| 6 | - |
reaction barley beta-glucan | Orpinomyces joyonii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | pH 4.5: about 15% of maximal activity, pH 5.0: about 95% of maximal activity, pH 8.5: about 50% of maximal activity, reaction with carboxymethyl cellulose | Orpinomyces joyonii |
| 5 | 7.5 | pH 5.0: about 75% of maximal activity, pH 7.5: about 50% of maximal activity, reaction with barley beta-glucan | Orpinomyces joyonii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.5 | 4°C, overnight, stable | Orpinomyces joyonii |
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