Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.4 extracted from

  • Li, D.C.; Lu, M.; Li, Y.L.; Lu, J.
    Purification and characterization of an endocellulase from the thermophilic fungus Chaetomium thermophilum CT2 (2003), Enzyme Microb. Technol., 33, 932-937.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Thermochaetoides thermophila
Ba2+
-
Thermochaetoides thermophila
Cu2+
-
Thermochaetoides thermophila
Fe2+
-
Thermochaetoides thermophila
Hg2+
-
Thermochaetoides thermophila
Mg2+
-
Thermochaetoides thermophila
Mn2+
-
Thermochaetoides thermophila
NH4+
-
Thermochaetoides thermophila
Zn2+
-
Thermochaetoides thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information the KM-value for the carboxymethylcellulose Na salt is 4.6 mg/ml Thermochaetoides thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ enhances activity Thermochaetoides thermophila
K+ enhances activity Thermochaetoides thermophila
Na+ enhances activity Thermochaetoides thermophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67800
-
-
Thermochaetoides thermophila

Organism

Organism UniProt Comment Textmining
Thermochaetoides thermophila
-
-
-
Thermochaetoides thermophila CT2
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 18.9% carbohydrate Thermochaetoides thermophila

Purification (Commentary)

Purification (Comment) Organism
-
Thermochaetoides thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylcellulose + H2O
-
Thermochaetoides thermophila ?
-
?
carboxymethylcellulose + H2O
-
Thermochaetoides thermophila CT2 ?
-
?
phosphoric acid-swollen cellulose + H2O low activity Thermochaetoides thermophila ?
-
?
phosphoric acid-swollen cellulose + H2O low activity Thermochaetoides thermophila CT2 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Thermochaetoides thermophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
1 h, stable Thermochaetoides thermophila
70
-
half-life: 45 min Thermochaetoides thermophila
80
-
half-life: 24 min Thermochaetoides thermophila
90
-
half-life: 7 min Thermochaetoides thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Thermochaetoides thermophila