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Literature summary for 3.2.1.4 extracted from

  • Hakamada, Y.; Endo, K.; Takizawa, S.; Kobayashi, T.; Shirai, T.; Yamane, T.; Ito, S.
    Enzymatic properties, crystallization, and deduced amino acid sequence of an alkaline endoglucanase from Bacillus circulans (2002), Biochim. Biophys. Acta, 1570, 174-180.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor-diffusion method with phosphate plus CdCl2 as precipitant. Pyramid-like crystals are formed and the diffract X-rays beyond 2.2 A resolution. It belongs to the space group P2(1)2(1)2(1) with unit cell parameters of a = 62.5 A, b = 71.7 A and c = 88.6 A Niallia circulans

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by EDTA, EGTA, 1,10-phenanthroline, monoiodoacetate, NEM, DTNB, PCMB and diethylpyrocarbonate Niallia circulans
N-bromosuccinimide 1 mM, 90% inhibition Niallia circulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km-value for carboxyymethylcellulose is 7.1 g/L at pH 8.5 and at 40°C Niallia circulans

Organism

Organism UniProt Comment Textmining
Niallia circulans
-
KSM-N257
-
Niallia circulans KSM-N257
-
KSM-N257
-

Purification (Commentary)

Purification (Comment) Organism
-
Niallia circulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.7
-
-
Niallia circulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylcellulose + H2O
-
Niallia circulans ?
-
?
carboxymethylcellulose + H2O
-
Niallia circulans KSM-N257 ?
-
?
cellohexaose + H2O
-
Niallia circulans 2 cellotriose + small amounts of cellobiose and cellotriose ?
cellohexaose + H2O
-
Niallia circulans KSM-N257 2 cellotriose + small amounts of cellobiose and cellotriose ?
cellopentaose + H2O
-
Niallia circulans cellobiose + cellotriose
-
?
cellopentaose + H2O
-
Niallia circulans KSM-N257 cellobiose + cellotriose
-
?
lichenan + H2O
-
Niallia circulans ?
-
?
lichenan + H2O
-
Niallia circulans KSM-N257 ?
-
?
additional information no hydrolysis of cellobioside, cellotrioside, cellotetraoside, laminarin, curdlan, xylan, p-nitrophenyl beta-D-cellotrioside and p-nitrophenyl beta-D-cellotetraoside Niallia circulans ?
-
?
additional information no hydrolysis of cellobioside, cellotrioside, cellotetraoside, laminarin, curdlan, xylan, p-nitrophenyl beta-D-cellotrioside and p-nitrophenyl beta-D-cellotetraoside Niallia circulans KSM-N257 ?
-
?

Synonyms

Synonyms Comment Organism
Egl-257
-
Niallia circulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
hydrolysis of carboxymethylcellulose Niallia circulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
pH 7.5, 15 min, stable up to Niallia circulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
26.4
-
carboxymethylcellulose pH 8.5, 40°C Niallia circulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
hydrolysis of carboxymethylcellulose Niallia circulans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 30°C, 1 h, stable Niallia circulans

pI Value

Organism Comment pI Value Maximum pI Value
Niallia circulans
-
4.7 4.6