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Literature summary for 3.2.1.4 extracted from
- Site of secretion and properties of endogenous endo-beta-1,4-glucanase components from Reticulitermes speratus (Kolbe), a Japanese subterranean termite (1997), Insect Biochem. Mol. Biol., 27, 305-313.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Reticulitermes speratus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
x * 41000, endo-beta-1,4-glucanase component YEG2, SDS-PAGE | Reticulitermes speratus |
| 42000 | - |
x * 42000, endo-beta-1,4-glucanase component YEG1, SDS-PAGE | Reticulitermes speratus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Reticulitermes speratus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| two endo-beta-1,4-glucanase components: YEG1 and YEG2 | Reticulitermes speratus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| salivary gland | site of secretion of endo-beta-1,4-glucanase | Reticulitermes speratus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 83.4 | - |
- |
Reticulitermes speratus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellopentaose + H2O | hydrolyzed by endo-beta-1,4-glucanase component YEG1 and YEG2 | Reticulitermes speratus | additional information | cellobiose + cellotriose | ? |  |
| cellotetraose + H2O | - |
Reticulitermes speratus | 2 cellobiose | - |
? | |
| cellotriose + H2O | hydrolyzed by endo-beta-1,4-glucanase component YEG2, no hydrolysis with endo-beta-1,4-glucanase component YEG1 | Reticulitermes speratus | D-glucose + cellobiose | + glucose | ? | |
| cellulose + H2O | - |
Reticulitermes speratus | ? | crystalline cellulose is hydrolyzed to glucose and cellobiose | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, endo-beta-1,4-glucanase component YEG1, SDS-PAGE | Reticulitermes speratus |
| ? | x * 41000, endo-beta-1,4-glucanase component YEG2, SDS-PAGE | Reticulitermes speratus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
30 min, stable up to | Reticulitermes speratus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Reticulitermes speratus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.9 | 7.4 | endo-beta-1,4-glucanase YEG2 retains over 80% of maximal activity from pH 4.9 to 7.4 | Reticulitermes speratus |
| 5.3 | 7 | endo-beta-1,4-glucanase YEG1 retains over 80% of maximal activity from pH 5.3 to 7.0 | Reticulitermes speratus |
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