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Literature summary for 3.2.1.4 extracted from
- Sclerotium rolfsii: status in cellulase research (1988), FEMS Microbiol. Rev., 54, 177-194.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Aspergillus niger | |
| additional information | - |
additional information | - |
Trichoderma reesei | |
| additional information | - |
additional information | - |
Athelia rolfsii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
1 * 22000 + 1 * 32000, SDS-PAGE | Athelia rolfsii |
| 28000 | - |
endoglucanase B | Athelia rolfsii |
| 32000 | - |
1 * 22000 + 1 * 32000, SDS-PAGE | Athelia rolfsii |
| 52000 | - |
endoglucanase A | Athelia rolfsii |
| 78000 | - |
endoglucanase C | Athelia rolfsii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Athelia rolfsii | - |
- |
- |
| Sporotrichum pulverulentum | - |
- |
- |
| Talaromyces funiculosus | - |
- |
- |
| Trichoderma reesei | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Aspergillus niger |
| glycoprotein | - |
Talaromyces funiculosus |
| glycoprotein | - |
Sporotrichum pulverulentum |
| glycoprotein | endoglucanase A, B and C are glycoproteins | Athelia rolfsii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellohexaose + H2O | - |
Aspergillus niger | additional information | - |
? |  |
| cellohexaose + H2O | - |
Athelia rolfsii | additional information | cellotriose + cellobiose + cellotriose | ? | |
| cellopentaose + H2O | - |
Aspergillus niger | additional information | - |
? | |
| cellopentaose + H2O | - |
Athelia rolfsii | additional information | cellobiose + cellotriose + glucose | ? | |
| cellotetraose + H2O | - |
Aspergillus niger | 2 cellobiose | - |
? | |
| cellotetraose + H2O | - |
Athelia rolfsii | 2 cellobiose | - |
? | |
| cellulose + H2O | - |
Aspergillus niger | ? | - |
? | |
| cellulose + H2O | - |
Trichoderma reesei | ? | - |
? | |
| cellulose + H2O | - |
Sporotrichum pulverulentum | ? | - |
? | |
| cellulose + H2O | Walseth cellulose | Athelia rolfsii | ? | - |
? | |
| cellulose + H2O | Walseth cellulose | Talaromyces funiculosus | ? | - |
? | |
| xylan + H2O | - |
Talaromyces funiculosus | additional information | - |
? | |
| xylan + H2O | hydrolysis with endoglucanase B, no action with endoglucanase A and C | Athelia rolfsii | additional information | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 22000 + 1 * 32000, SDS-PAGE | Athelia rolfsii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Talaromyces funiculosus |
| 50 | - |
endoglucanase B and C | Athelia rolfsii |
| 74 | - |
endoglucanase A | Athelia rolfsii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Aspergillus niger |
| 70 | - |
10 min, 20% loss of activity | Athelia rolfsii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.8 | - |
and a second optimum at pH 3.2, endoglucanase B | Athelia rolfsii |
| 3.2 | - |
and a second optimum at pH 2.8, endoglucanase B | Athelia rolfsii |
| 4 | - |
- |
Aspergillus niger |
| 4 | - |
endoglucanase A and C | Athelia rolfsii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
stable, endoglucanase A, B and C | Athelia rolfsii |
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