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Literature summary for 3.2.1.39 extracted from

  • Hantani, Y.; Motoki, S.; Miyagawa, A.; Yamamura, H.; Oda, M.
    Transglycosylation activity of catalytic domain mutant of endo-1,3-beta-glucanase from Cellulosimicrobium cellulans (2018), Protein Pept. Lett., 25, 734-739 .
    View publication on PubMed
Search for more literature for 3.2.1.39

Protein Variants

Protein Variants Comment Organism
E119G site-directed mutagenesis, glucosidase inactive catalytic residue mutant, the mutant shows transglycosylation activity toward laminarioligosaccharides. The hydrolytic as well as transglycosylation activities of E119G decrease with the decrease in temperature, but the ratio of transglycosylation products increases. The enzymatic activity of E119G toward laminaritriose in the presence of glucose is abolished, while the addition of laminaribiose evidently increases the transglycosylation products such as laminaritetraose and laminaripentaose Cellulosimicrobium cellulans

Organism

Organism UniProt Comment Textmining
Cellulosimicrobium cellulans P22222 i.e. Arthrobacter luteus
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Cellulosimicrobium cellulans DK-1 P22222 i.e. Arthrobacter luteus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
laminaribiose + H2O wild-type enzyme performs glucosidase activity on laminarioligosaccharides, while the mutant performs transglycosylation Cellulosimicrobium cellulans ?
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 ?
laminaribiose + H2O wild-type enzyme performs glucosidase activity on laminarioligosaccharides, while the mutant performs transglycosylation Cellulosimicrobium cellulans DK-1 ?
-
 ?
laminaritriose + H2O wild-type enzyme performs glucosidase activity on laminarioligosaccharides, while the mutant performs transglycosylation Cellulosimicrobium cellulans ?
-
 ?
laminaritriose + H2O wild-type enzyme performs glucosidase activity on laminarioligosaccharides, while the mutant performs transglycosylation Cellulosimicrobium cellulans DK-1 ?
-
 ?

Synonyms

Synonyms Comment Organism
endo-1,3-beta-glucanase
-
 Cellulosimicrobium cellulans

pH Range

pH Minimum pH Maximum Comment Organism
4 8.5 activity range, lower activity at pH 8.0-8.5 Cellulosimicrobium cellulans

General Information

General Information Comment Organism
malfunction substitution of catalytic residue E119 in glycosidase results in the loss of hydrolytic activity Cellulosimicrobium cellulans
additional information residue Glu119 acts as a nucleophile in the endo-1,3-beta-glucanase reaction and has an effect on the balance between hydrolysis and transglycosylation Cellulosimicrobium cellulans