Any feedback?
Literature summary for 3.2.1.39 extracted from
- A new recombinant endo-1,3-beta-D-glucanase from the marine bacterium Formosa algae KMM 3553 enzyme characteristics and transglycosylation products analysis (2017), World J. Microbiol. Biotechnol., 33, 40 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta (DE3) cells | Formosa algae |
| expression in Escherichia coli | Formosa algae |
General Stability
| General Stability | Organism |
|---|---|
| 62% of activity is retained at 2.0 M NaCl | Formosa algae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.388 | - |
Laminarin | at pH 5.4 and 37°C | Formosa algae |  |
| 0.39 | - |
Laminarin | pH 6.0, 37°C | Formosa algae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Formosa algae | - |
- |
- |
| Formosa algae | A0A0B5GQL6 | - |
- |
| Formosa algae KMM 3553 | - |
- |
- |
| Formosa algae KMM 3553 | A0A0B5GQL6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose column chromatography, Ni-NTA agarose column chromatography, and Sephacryl S-100 gel filtration | Formosa algae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 24 | - |
pH 6.0 37°C | Formosa algae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethyl-pachyman + H2O | 1.7% activity compared to laminarin | Formosa algae | ? | - |
? | |
| laminarin + H2O | 100% activity | Formosa algae | ? | - |
? | |
| laminarin + H2O | 100% activity | Formosa algae KMM 3553 | ? | - |
? | |
| laminarin + H2O | laminarin from Saccharina cichorioides with molecular weight of 3.5 kDa | Formosa algae | laminaritrisaccharides + laminaritetrasaccharides | - |
? | |
| laminarin + H2O | laminarin from Saccharina cichorioides with molecular weight of 3.5 kDa | Formosa algae KMM 3553 | laminaritrisaccharides + laminaritetrasaccharides | - |
? | |
| lichenan + H2O | 1.1% activity compared to laminarin | Formosa algae | ? | - |
? | |
| lichenan + H2O | 1.1% activity compared to laminarin | Formosa algae KMM 3553 | ? | - |
? | |
| additional information | enzyme catalyzes transfer of the glyconic part of a substrate molecule on methyl-beta-D-xylopyranoside, glycerol and methyl-alpha-D-glucopyranoside. Low activity on yeast glucan, no substrates: pachyman and aubasidan, pustulan, pullulan, amylopectin, and carboxymethylcellulose | Formosa algae | ? | - |
? | |
| additional information | the enzyme catalyzes transfer of glyconic part of substrate molecule on methyl-beta-D-xylopyranoside, glycerol and methyl-alpha-D-glucopyranoside | Formosa algae | ? | - |
? | |
| additional information | the enzyme has no ability to degrade pachiman, aubasidan, pustulan, pullulan, amylopectin, and carboxymethyl-cellulose | Formosa algae | ? | - |
? | |
| additional information | the enzyme catalyzes transfer of glyconic part of substrate molecule on methyl-beta-D-xylopyranoside, glycerol and methyl-alpha-D-glucopyranoside | Formosa algae KMM 3553 | ? | - |
? | |
| additional information | the enzyme has no ability to degrade pachiman, aubasidan, pustulan, pullulan, amylopectin, and carboxymethyl-cellulose | Formosa algae KMM 3553 | ? | - |
? | |
| additional information | enzyme catalyzes transfer of the glyconic part of a substrate molecule on methyl-beta-D-xylopyranoside, glycerol and methyl-alpha-D-glucopyranoside. Low activity on yeast glucan, no substrates: pachyman and aubasidan, pustulan, pullulan, amylopectin, and carboxymethylcellulose | Formosa algae KMM 3553 | ? | - |
? | |
| periodate-oxidized laminarin + H2O | - |
Formosa algae | ? | - |
? | |
| periodate-oxidized laminarin + H2O | 98% activity compared to laminarin | Formosa algae | ? | - |
? | |
| periodate-oxidized laminarin + H2O | 98% activity compared to laminarin | Formosa algae KMM 3553 | ? | - |
? | |
| periodate-oxidized laminarin + H2O | - |
Formosa algae KMM 3553 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 61000, SDS-PAGE | Formosa algae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-1,3-beta-D-glucanase | - |
Formosa algae |
| Gfa | - |
Formosa algae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Formosa algae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 50 | the enzyme shows about 50% activity at 30°C, about 60% activity at 35°C, about 80% activity at 40°C, 100% activity at 45°C, about 30% activity at 50°C, and no activity at 55°C and above | Formosa algae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
half-life 20 min | Formosa algae |
| 45 | 55 | the half-life period at 45°C is 20 min, and complete inactivation happens at 55°C within 10 min | Formosa algae |
| 55 | - |
10 min, complete loss of activity | Formosa algae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Formosa algae |
| 5.5 | 6 | - |
Formosa algae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | the enzyme shows more than 60% activity between pH 4.0 and 8.0 | Formosa algae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
