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Literature summary for 3.2.1.39 extracted from

  • Mestre, P.; Arista, G.; Piron, M.C.; Rustenholz, C.; Ritzenthaler, C.; Merdinoglu, D.; Chich, J.F.
    Identification of a Vitis vinifera endo-beta-1,3-glucanase with antimicrobial activity against Plasmopara viticola (2017), Mol. Plant Pathol., 18, 708-719 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.39

Application

Application Comment Organism
agriculture isoform EGase3 shows strong antimicrobial activity against germinated spores of Plasmopara viticola Vitis vinifera

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Vitis vinifera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.081
-
 Laminarin pH 5.4, 37°C Vitis vinifera
0.147
-
 Laminarin pH 5.4, 37°C Vitis vinifera
0.228
-
 Laminarin pH 5.4, 37°C Vitis vinifera

Organism

Organism UniProt Comment Textmining
Vitis vinifera A7PQW3 isoform EGase2
-
Vitis vinifera F6HLL8 isoform EGase3
-
Vitis vinifera Q9M563 isoform EGase1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
laminarin + H2O
-
 Vitis vinifera ?
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE, x * 37100, calculated Vitis vinifera
? x * 39000, SDS-PAGE, x * 38500, calculated Vitis vinifera

Synonyms

Synonyms Comment Organism
EGase1
-
 Vitis vinifera
EGase2
-
 Vitis vinifera
EGase3
-
 Vitis vinifera
VIT_205s0077g01150
-
 Vitis vinifera
VIT_206s0061g00120
-
 Vitis vinifera
VIT_208s0007g06060
-
 Vitis vinifera

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Vitis vinifera

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
 Laminarin pH 5.4, 37°C Vitis vinifera
34
-
 Laminarin pH 5.4, 37°C Vitis vinifera
92
-
 Laminarin pH 5.4, 37°C Vitis vinifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Vitis vinifera
5.5
-
-
 Vitis vinifera

pI Value

Organism Comment pI Value Maximum pI Value
Vitis vinifera calculated
-
 4.9
Vitis vinifera calculated
-
 9.4
Vitis vinifera calculated
-
 9.5

Expression

Organism Comment Expression
Vitis vinifera expression is induced by Plasmapora viticola infection up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
40
-
 Laminarin pH 5.4, 37°C Vitis vinifera
230
-
 Laminarin pH 5.4, 37°C Vitis vinifera
400
-
 Laminarin pH 5.4, 37°C Vitis vinifera