Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus tequilensis |
Protein Variants | Comment | Organism |
---|---|---|
K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P | the mutant shows 63% increased catalytic activity and stability compared to the wild type enzyme. Half-life values at 60°C and 70°C are 152 and 99 min, respectively | Bacillus tequilensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 41% residual activity at 10 mM | Bacillus tequilensis | |
Ca2+ | 84% residual activity at 10 mM | Bacillus tequilensis | |
Co2+ | 94% residual activity at 10 mM | Bacillus tequilensis | |
Cu2+ | 86% residual activity at 10 mM | Bacillus tequilensis | |
dithiothreitol | 35% residual activity at 10 mM | Bacillus tequilensis | |
EDTA | 31% residual activity at 10 mM | Bacillus tequilensis | |
Fe2+ | 8.4% residual activity at 10 mM | Bacillus tequilensis | |
Fe3+ | 6.5% residual activity at 10 mM | Bacillus tequilensis | |
Li+ | 51% residual activity at 10 mM | Bacillus tequilensis | |
Mg2+ | 50% residual activity at 10 mM | Bacillus tequilensis | |
Mn2+ | 55% residual activity at 10 mM | Bacillus tequilensis | |
Zn2+ | 35% residual activity at 10 mM | Bacillus tequilensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
Barley beta-glucan | mutant enzyme K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, at pH 6.5 and 40°C | Bacillus tequilensis | |
0.3 | - |
Barley beta-glucan | wild type enzyme, at pH 6.5 and 40°C | Bacillus tequilensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not influenced by K+, Na+, or NH4+ | Bacillus tequilensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus tequilensis | - |
- |
- |
Bacillus tequilensis CGX 5-2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Bacillus tequilensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | - |
Bacillus tequilensis | ? | - |
? | |
barley beta-glucan + H2O | - |
Bacillus tequilensis CGX 5-2 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Bgl | - |
Bacillus tequilensis |
endo-beta-1,3-glucanase | - |
Bacillus tequilensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bacillus tequilensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 55 | more than 70% activity between 35 and 55°C | Bacillus tequilensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | half-life values at 60°C and 70°C are 32.5 and 11 min, respectively. The enzyme is almost fully inactivated after incubation at 60°C for 140 min | Bacillus tequilensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
137 | - |
Barley beta-glucan | wild type enzyme, at pH 6.5 and 40°C | Bacillus tequilensis | |
189 | - |
Barley beta-glucan | mutant enzyme K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, at pH 6.5 and 40°C | Bacillus tequilensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
462 | - |
Barley beta-glucan | wild type enzyme, at pH 6.5 and 40°C | Bacillus tequilensis | |
691 | - |
Barley beta-glucan | mutant enzyme K20S/N31C/S40E/S43E/E46P/P102C/K117S/N125C/K165S/T187C/H205P, at pH 6.5 and 40°C | Bacillus tequilensis |