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Literature summary for 3.2.1.39 extracted from
- Modulating the function of a beta-1,3-glucanosyltransferase to that of an endo-beta-1,3-glucanase by structure-based protein engineering (2016), Appl. Microbiol. Biotechnol., 100, 1765-1776 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Rhizomucor miehei |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E158A | mutation switches the activity from mainly transglycosylation to beta-1,3-glucanase. Hydrolytic activity toward reduced laminarin is 348.5fold higher than the wild type | Rhizomucor miehei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizomucor miehei | - |
- |
- |
| Rhizomucor miehei | A0A0K0M737 | - |
- |
| Rhizomucor miehei CAU432 | - |
- |
- |
| Rhizomucor miehei CAU432 | A0A0K0M737 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-IDA column chromatography, and Sephacryl S-100 gel filtration | Rhizomucor miehei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.115 | - |
wild-type, beta-1,3-glucanase activity, pH 5.0, 50°C | Rhizomucor miehei |
| 2.45 | - |
wild-type, transglycosylation activity, pH 5.0, 50°C | Rhizomucor miehei |
| 39.95 | - |
mutant E158A, beta-1,3-glucanase activity, pH 5.0, 50°C | Rhizomucor miehei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 laminaripentaose + H2O | transglycosylation activity, product is a linear beta-1,3-octasaccharide containing an intrachain beta-1,6 linkage | Rhizomucor miehei | octasaccharide + ? | - |
? |  |
| 2 laminaripentaose + H2O | transglycosylation activity, product is a linear beta-1,3-octasaccharide containing an intrachain beta-1,6 linkage | Rhizomucor miehei CAU432 | octasaccharide + ? | - |
? | |
| curdlan + H2O | - |
Rhizomucor miehei | laminaribiose + laminaritriose | - |
? | |
| curdlan + H2O | - |
Rhizomucor miehei CAU432 | laminaribiose + laminaritriose | - |
? | |
| laminaripentaose + H2O | efficient hydrolytic activity | Rhizomucor miehei | laminaritetraose + ? | - |
? | |
| laminaripentaose + H2O | efficient hydrolytic activity | Rhizomucor miehei CAU432 | laminaritetraose + ? | - |
? | |
| laminaritetraose + H2O | efficient hydrolytic activity | Rhizomucor miehei | laminaritriose + ? | - |
? | |
| laminaritetraose + H2O | efficient hydrolytic activity | Rhizomucor miehei CAU432 | laminaritriose + ? | - |
? | |
| additional information | the enzyme hardly cleaves the beta-1,3 linkage in laminaribiose | Rhizomucor miehei | ? | - |
? | |
| additional information | the enzyme hardly cleaves the beta-1,3 linkage in laminaribiose | Rhizomucor miehei CAU432 | ? | - |
? | |
| reduced laminarin + H2O | - |
Rhizomucor miehei | laminarioligosaccharides | - |
? | |
| reduced laminarin + H2O | - |
Rhizomucor miehei CAU432 | laminarioligosaccharides | - |
? | |
| reduced laminarin + H2O | high hydrolytic activity | Rhizomucor miehei | laminaritriose + laminaritetraose + laminari-oligosaccharides | - |
? | |
| reduced laminarin + H2O | high hydrolytic activity | Rhizomucor miehei CAU432 | laminaritriose + laminaritetraose + laminari-oligosaccharides | - |
? | |
| yeast beta-D-glucan + H2O | - |
Rhizomucor miehei | laminaritriose + laminari-oligosaccharides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31000, SDS-PAGE | Rhizomucor miehei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Bgt17AE158A | E158A mutant of family 17 beta-1,3-glucanosyltransferase | Rhizomucor miehei |
| endo-beta-1,3-glucanase | - |
Rhizomucor miehei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Rhizomucor miehei |
| 55 | - |
mutant E158A | Rhizomucor miehei |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | the enzyme retains over 50 % of its activity between 40 and 60°C | Rhizomucor miehei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
the enzyme is stable up to 50 °C, with more than 90 % of its activity remaining after 30 min | Rhizomucor miehei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Rhizomucor miehei |
| 4.5 | - |
mutant E158A | Rhizomucor miehei |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | the enzyme exhibits good pH stability within the range of pH 4.0-8.0, retaining over 80 % of its activity after 30 min | Rhizomucor miehei |
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