Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli using the T7-expression system | Pyrococcus furiosus |
Protein Variants | Comment | Organism |
---|---|---|
delMet174 | the specific activities of the mutant LamA, on both laminarin and lichenan, is about 10fold lower than the respective wild-type value. The methionine deletion leads to an enzyme with 13.1% of wild-type Vmax and a slightly higher Km on laminarin as a substrate. With lichenan as substrate, the mutant LamA has 7.9% of the wild-type Vmax and the same Km value | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
Laminarin | 2.8 mg/ml, pH 6.5, 80°C, wild-type enzyme. 3.5 mg/ml, pH 6.5, 80°C, mutant enzyme delMet174 | Pyrococcus furiosus | |
additional information | - |
lichenan | 4.7 mg/ml, pH 6.5, 80°C, wild-type enzyme. 4.9 mg/ml, pH 6.5, 80°C, mutant enzyme delMet174 | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | O73951 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | main linkage type beta-1,3-1,4(Glc), about 10% of the specific activity compared to laminarin | Pyrococcus furiosus | ? | - |
? | |
laminarin + H2O | main linkage type beta-1,3-(Glc). The enzyme cleaves laminarin randomly, yielding glucose, laminaribiose, and higher laminari-oligosaccharides | Pyrococcus furiosus | ? | - |
? | |
lichenan + H2O | main linkage type beta-1,3-1,4(Glc), about 10% of the specific activity compared to laminarin | Pyrococcus furiosus | ? | - |
? | |
additional information | no activity with cellulose, xylan and chitin | Pyrococcus furiosus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-1,3-glucanase | - |
Pyrococcus furiosus |
LamA | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Pyrococcus furiosus |
100 | 105 | - |
Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 115 | 80°C: about 50% of maximal activity, 115°C: about 30% of maximal activity | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
110 h, stable | Pyrococcus furiosus |
90 | - |
half-life: 64 h | Pyrococcus furiosus |
100 | - |
half-life: 19 h | Pyrococcus furiosus |
110 | - |
half-life: 15 min | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | - |
Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8 | pH 4.5: about 75% of maximal activity, pH 8.0: about 55% of maximal activity | Pyrococcus furiosus |