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Literature summary for 3.2.1.39 extracted from

  • Sakamoto, Y.; Nakade, K.; Konno, N.
    An endo-{beta}-1,3-glucanase, GLU1, from Lentinula edodes fruiting body belongs to a new glycoside hydrolase family (2011), Appl. Environ. Microbiol., 77, 8350-8354.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Lentinula edodes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
x * 25000, SDS-PAGE, x * 26000, calculated Lentinula edodes
26000
-
x * 25000, SDS-PAGE, x * 26000, calculated Lentinula edodes

Organism

Organism UniProt Comment Textmining
Lentinula edodes G9M5R4 Glu1 does not belong to existing GH families containing EC 3.2.1.39 glucanases
-

Purification (Commentary)

Purification (Comment) Organism
from fruiting body Lentinula edodes

Source Tissue

Source Tissue Comment Organism Textmining
fruit body
-
Lentinula edodes
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.85
-
pH 4.2, 37°C Lentinula edodes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl curdlan + H2O 13.7% of the activity with laminarin Lentinula edodes ?
-
?
carboxymethyl pachyman + H2O 9.1% of the activity with laminarin Lentinula edodes ?
-
?
curdlan + H2O 9.1% of the activity with laminarin Lentinula edodes ?
-
?
laminarin + H2O endolytic depolymerization of glucans composed of beta-1,3-linked main chains Lentinula edodes D-glucose + laminarioligosaccharides
-
?
lentinan + H2O 4.4% of the activity with laminarin Lentinula edodes ?
-
?
additional information no substrate: beta-1,3-1,4-glucans such as barley glucan or lichenan, or beta-1,6 glucans such as pustulan Lentinula edodes ?
-
?
pachyman + H2O 14.9% of the activity with laminarin Lentinula edodes ?
-
?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE, x * 26000, calculated Lentinula edodes

Synonyms

Synonyms Comment Organism
Glu1
-
Lentinula edodes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Lentinula edodes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
30 min, complete inactivation Lentinula edodes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.84
-
Laminarin pH 4.2, 37°C Lentinula edodes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Lentinula edodes

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 4°C, stable for 20 h Lentinula edodes

pI Value

Organism Comment pI Value Maximum pI Value
Lentinula edodes isoelectric focusing
-
3.9