Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Lentinula edodes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 25000, SDS-PAGE, x * 26000, calculated | Lentinula edodes |
26000 | - |
x * 25000, SDS-PAGE, x * 26000, calculated | Lentinula edodes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lentinula edodes | G9M5R4 | Glu1 does not belong to existing GH families containing EC 3.2.1.39 glucanases | - |
Purification (Comment) | Organism |
---|---|
from fruiting body | Lentinula edodes |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit body | - |
Lentinula edodes | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.85 | - |
pH 4.2, 37°C | Lentinula edodes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl curdlan + H2O | 13.7% of the activity with laminarin | Lentinula edodes | ? | - |
? | |
carboxymethyl pachyman + H2O | 9.1% of the activity with laminarin | Lentinula edodes | ? | - |
? | |
curdlan + H2O | 9.1% of the activity with laminarin | Lentinula edodes | ? | - |
? | |
laminarin + H2O | endolytic depolymerization of glucans composed of beta-1,3-linked main chains | Lentinula edodes | D-glucose + laminarioligosaccharides | - |
? | |
lentinan + H2O | 4.4% of the activity with laminarin | Lentinula edodes | ? | - |
? | |
additional information | no substrate: beta-1,3-1,4-glucans such as barley glucan or lichenan, or beta-1,6 glucans such as pustulan | Lentinula edodes | ? | - |
? | |
pachyman + H2O | 14.9% of the activity with laminarin | Lentinula edodes | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, SDS-PAGE, x * 26000, calculated | Lentinula edodes |
Synonyms | Comment | Organism |
---|---|---|
Glu1 | - |
Lentinula edodes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Lentinula edodes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
30 min, complete inactivation | Lentinula edodes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.84 | - |
Laminarin | pH 4.2, 37°C | Lentinula edodes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Lentinula edodes |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | 4°C, stable for 20 h | Lentinula edodes |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Lentinula edodes | isoelectric focusing | - |
3.9 |