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Literature summary for 3.2.1.39 extracted from
- Purification, characterization and sequencing of the major beta-1,3-glucanase from the midgut of Tenebrio molitor larvae (2009), Insect Biochem. Mol. Biol., 39, 861-874.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| TLam DNA and amino acid sequence determination and analysis, phylogenetic analysisand tree | Tenebrio molitor |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is not inhibited by high substrate concentrations | Tenebrio molitor |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, overview | Tenebrio molitor |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Tenebrio molitor | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40117 | - |
x * 50000, SDS-PAGE, x * 40117, sequence calculation | Tenebrio molitor |
| 50000 | - |
x * 50000, SDS-PAGE, x * 40117, sequence calculation | Tenebrio molitor |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Tenebrio molitor | the enzyme is an endoglucanase that hydrolyzes beta-1,3-glucans as laminarin and yeast beta-1,3-1,6-glucan, but is inactive toward other polysaccharides, e.g. unbranched beta-1,3-glucans or mixed beta-1,3-1,4-glucan from cereals, or disaccharides | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tenebrio molitor | C6GFH7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 113fold from midgut to homogeneity by anion exchange and hydrophobic interaction chromatography, and gel filtration | Tenebrio molitor |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| laminariheptaose + 3 H2O = laminaritriose + 2 laminaribiose | the enzyme is an endoglucanase that hydrolyzes beta-1,3-glucans as laminarin and yeast beta-1,3-1,6-glucan, but is inactive toward other polysaccharides, e.g. unbranched beta-1,3-glucans or mixed beta-1,3-1,4-glucan from cereals, or disaccharides. TLam has two ionizable groups involved in catalysis, and His, Tyr and Arg residues plus a divalent ion at the active site. A Cys residue important for TLam activity is exposed after laminarin binding. Residues E174, E179, H204, Y304, R127 and R181 at the active site of the enzyme are important for TLam activity | Tenebrio molitor |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gut | - |
Tenebrio molitor | - |
| larva | - |
Tenebrio molitor | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.4 | - |
purified native enzyme | Tenebrio molitor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| avicel + H2O | microcrystalline cellulose | Tenebrio molitor | ? | - |
? | |
| beta-1,3-1,4-glucan + H2O | substrate from Hordeum vulgare, substrate of EC 3.2.1.6, endo-1,3(4)-beta-glucanase | Tenebrio molitor | ? | - |
? | |
| carboxymethylcellulose + H2O | - |
Tenebrio molitor | ? | - |
? | |
| laminarin + H2O | substrate from Laminaria digitata | Tenebrio molitor | ? | - |
? | |
| lichenan + H2O | substrate from Cetraria islandica | Tenebrio molitor | ? | - |
? | |
| additional information | the enzyme is an endoglucanase that hydrolyzes beta-1,3-glucans as laminarin and yeast beta-1,3-1,6-glucan, but is inactive toward other polysaccharides, e.g. unbranched beta-1,3-glucans or mixed beta-1,3-1,4-glucan from cereals, or disaccharides | Tenebrio molitor | ? | - |
? | |
| pachyman + H2O | substrate from Poria cocos | Tenebrio molitor | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, SDS-PAGE, x * 40117, sequence calculation | Tenebrio molitor |
| More | homology model of the TLam structure, overview | Tenebrio molitor |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-1,3-glucanase | - |
Tenebrio molitor |
| laminarinase | - |
Tenebrio molitor |
| More | the enzyme belongs to glycoside hydrolase family 16 | Tenebrio molitor |
| TLam | - |
Tenebrio molitor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Tenebrio molitor |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
Laminarin | pH 6.0, 30°C | Tenebrio molitor | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Tenebrio molitor |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH effects on native and chemically modified enzyme, profiles, overview | Tenebrio molitor |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Tenebrio molitor | TLam, sequence calculation | - |
4.33 |
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