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Literature summary for 3.2.1.39 extracted from

  • Kovalchuk, S.N.; Sundukova, E.V.; Kusaykin, M.I.; Guzev, K.V.; Anastiuk, S.D.; Likhatskaya, G.N.; Trifonov, E.V.; Nurminski, E.A.; Kozhemyako, V.B.; Zvyagintseva, T.N.; Rasskazov, V.A.
    Purification, cDNA cloning and homology modeling of endo-1,3-beta-D-glucanase from scallop Mizuhopecten yessoensis (2006), Comp. Biochem. Physiol. B, 143B, 473-485.
No PubMed abstract available
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Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-3(-dimethylaminopropyl)carbodiimide 85% residual activity at 10 mM (pH 5.0) Mizuhopecten yessoensis
diethyl dicarbonate complete loss of activity at 36 mM (pH 6.0) Mizuhopecten yessoensis
additional information not inhibited by EDTA, p-chloromercuribenzoate, iodoacetamide, N-ethylmaleimide, Ellman's reagent, N-acetylimidazole, tetranitromethane, 2,4-pentanedione, and trinitrobenzolsulfonic acid Mizuhopecten yessoensis
N-bromosuccinimide complete loss of activity at 0.1 mM (pH 4.0) Mizuhopecten yessoensis
propylene oxide 15% residual activity at 6 M (pH 4.0) Mizuhopecten yessoensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 SDS-PAGE Mizuhopecten yessoensis
36900
-
 estimated from amino acid sequence Mizuhopecten yessoensis

Organism

Organism UniProt Comment Textmining
Mizuhopecten yessoensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
CM-Sephadex C-50 gel filtration, phenyl-Sepharose CL-4B column chromatography, and Sephadex G-75 gel filtration Mizuhopecten yessoensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.095
-
 crude extract Mizuhopecten yessoensis
6
-
 after 65fold purification Mizuhopecten yessoensis

Storage Stability

Storage Stability Organism
25°C, 0.05 M sodium acetate buffer at pH 4.5 with 0.25 M sodium chloride, 1 month, no loss of activity Mizuhopecten yessoensis
37°C, 0.05 M sodium acetate buffer at pH 4.5 with 0.25 M sodium chloride, 2 h, complete loss of activity Mizuhopecten yessoensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
laminarin + H2O
-
 Mizuhopecten yessoensis D-glucose + laminaribiose + ?
-
 ?

Synonyms

Synonyms Comment Organism
endo-1,3-beta-D-glucanase
-
 Mizuhopecten yessoensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 45
-
 Mizuhopecten yessoensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 rapid denaturation occurs above 45°C Mizuhopecten yessoensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Mizuhopecten yessoensis

pH Range

pH Minimum pH Maximum Comment Organism
4 5
-
 Mizuhopecten yessoensis