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Literature summary for 3.2.1.39 extracted from

  • Yamamoto, S.; Nagasaki, S.
    Purification, crystallization, and properties of an endo beta-1,3-glucanase from Rhizopus chinensis R-69 (1975), Agric. Biol. Chem., 39, 2163-2169.
No PubMed abstract available
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Inhibitors

Inhibitors Comment Organism Structure
AgNO3 complete inhibition at 1 mM Rhizopus microsporus var. chinensis
Ca2+ slight inhibition at 1 mM Rhizopus microsporus var. chinensis
Co2+ slight inhibition at 1 mM Rhizopus microsporus var. chinensis
Cr2(SO4)3 36% inhibition at 1 mM Rhizopus microsporus var. chinensis
Cu2+ 55% inhibition at 1 mM Rhizopus microsporus var. chinensis
Hg2+ marked inhibition at 1 mM Rhizopus microsporus var. chinensis
KBr slight inhibition at 1 mM Rhizopus microsporus var. chinensis
Mg2+ slight inhibition at 1 mM Rhizopus microsporus var. chinensis
Na2WO4 36% inhibition at 1 mM Rhizopus microsporus var. chinensis
NaAsO2 39% inhibition at 1 mM Rhizopus microsporus var. chinensis
Pb2+ 45% inhibition at 1 mM Rhizopus microsporus var. chinensis
Sn2+ 25% inhibition at 1 mM Rhizopus microsporus var. chinensis
Zn2+ 59% inhibition at 1 mM Rhizopus microsporus var. chinensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 Laminarin value in mg/ml Rhizopus microsporus var. chinensis
additional information
-
 Laminarin 3.4 Rhizopus microsporus var. chinensis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Rhizopus microsporus var. chinensis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 224% activation at 1 mM Rhizopus microsporus var. chinensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 gel filtration Rhizopus microsporus var. chinensis

Organism

Organism UniProt Comment Textmining
Rhizopus microsporus var. chinensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
in crystalline form Rhizopus microsporus var. chinensis

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Rhizopus microsporus var. chinensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4800
-
-
 Rhizopus microsporus var. chinensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-glucan + H2O low activity Rhizopus microsporus var. chinensis ?
-
 ?
beta-glucan + H2O from yeast Rhizopus microsporus var. chinensis ?
-
 ?
laminarin + H2O
-
 Rhizopus microsporus var. chinensis D-glucose + ?
-
 ?
pachyman + H2O low activity Rhizopus microsporus var. chinensis ?
-
 ?