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Literature summary for 3.2.1.39 extracted from
- Partial purification and properties of an endo-1,3-beta-D-glucanase from germinated rye (1978), Phytochemistry, 17, 1539-1543.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | marked stimulation dependent on buffer concentration, especially at low concentration | Secale cereale |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
Laminarin | value in mg/ml | Secale cereale |  |
| additional information | - |
Laminarin | 0.25 | Secale cereale | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | nearly 3fold activation at 5 mM | Secale cereale | |
| Mg2+ | 3.5fold activation at 5 mM | Secale cereale | |
| Na+ | slight activation at 5 mM | Secale cereale | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24300 | - |
gel filtration | Secale cereale |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Secale cereale | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Secale cereale |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | germinating grain | Secale cereale | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-O-beta-D-Glc-D-Glc-D-Glc + H2O | smallest substrate, products are smaller oligosaccharides and glucose | Secale cereale | ? | - |
? | |
| 3-O-beta-D-Glc-D-Glc-D-Glc + H2O | laminaritriose | Secale cereale | ? | - |
? | |
| 3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc + H2O | products are smaller oligosaccharides and glucose | Secale cereale | ? | - |
? | |
| 3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc + H2O | laminaritetraose | Secale cereale | ? | - |
? | |
| laminarin + H2O | preferred substrate | Secale cereale | D-glucose + ? | - |
? | |
| O-carboxymethyl-pachyman + H2O | preferred substrate | Secale cereale | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Secale cereale |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
maximum heat stability | Secale cereale |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
hydrolysis of laminarin | Secale cereale |
| 5.7 | - |
hydrolysis of CM-pachyman | Secale cereale |
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Release 2023.1 (January 2023)
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