Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.39 extracted from

  • Iwamuro, Y.; Aoki, M.; Mikami, Y.
    Purification and some properties of an endo-beta-1,3-glucanase from Porodisculus pendulus (1985), J. Ferment. Technol., 63, 61-66.
No PubMed abstract available
Search for more literature for 3.2.1.39

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ inhibition at 1 mM Porodisculus pendulus
Hg2+ marked inhibition at 1 mM Porodisculus pendulus
Pb2+ inhibition at 1 mM Porodisculus pendulus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 Laminarin value in mg/ml Porodisculus pendulus
additional information
-
 Laminarin 3.04 Porodisculus pendulus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Porodisculus pendulus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000 43000 SDS-PAGE, gel filtration Porodisculus pendulus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pendulan + H2O Porodisculus pendulus acts in random fashion, reaction product with high molecular weight, major role in decreasing the molecular weight of pendulan during fermentation reducing sugar + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Porodisculus pendulus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 5step chromatography Porodisculus pendulus

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Porodisculus pendulus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
70.8
-
-
 Porodisculus pendulus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc + H2O end-product laminaribiose, no glucose release Porodisculus pendulus ?
-
 ?
3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc + H2O laminaritetraose Porodisculus pendulus ?
-
 ?
3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc-D-Glc + H2O end-products laminaritriose and laminaribiose, no glucose release Porodisculus pendulus ?
-
 ?
3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc-D-Glc + H2O laminaripentaose Porodisculus pendulus ?
-
 ?
3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc-D-Glc-D-Glc + H2O main product laminaritriose, small amounts laminaritriose and laminaribiose, no glucose release Porodisculus pendulus ?
-
 ?
3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc-D-Glc-D-Glc + H2O laminarihexaose Porodisculus pendulus ?
-
 ?
curdlan + H2O
-
 Porodisculus pendulus ?
-
 ?
laminarin + H2O
-
 Porodisculus pendulus D-glucose + ?
-
 ?
pendulan + H2O acts in random fashion, reaction product with high molecular weight, major role in decreasing the molecular weight of pendulan during fermentation Porodisculus pendulus reducing sugar + ?
-
 ?
schizophyllan + H2O low activity Porodisculus pendulus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 42000-43000, SDS-PAGE, gel filtration Porodisculus pendulus

Synonyms

Synonyms Comment Organism
endo-beta-1,3-glucanase
-
 Porodisculus pendulus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Porodisculus pendulus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 stable below Porodisculus pendulus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Porodisculus pendulus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 7.3 stable within Porodisculus pendulus