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Literature summary for 3.2.1.39 extracted from
- A novel endo-beta-1,3-glucanase, BGN13.1, involved in the mycoparasitism of Trichoderma harzianum (1995), J. Bacteriol., 177, 6937-6945.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cDNA for sequence analysis | Trichoderma harzianum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
Laminarin | value in mg/ml | Trichoderma harzianum |  |
| additional information | - |
Laminarin | 3.3 | Trichoderma harzianum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Trichoderma harzianum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
SDS-PAGE | Trichoderma harzianum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cell wall glucan + H2O | Trichoderma harzianum | from yeast and different fungi, slight activity, involvement in mycoparasitism | reducing sugar + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma harzianum | - |
enzyme synthesis repressed by glucose, induced by fungal cell wall polymers or autoclaved mycelia from fungi | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, 2step chromatography | Trichoderma harzianum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Trichoderma harzianum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Trichoderma harzianum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-glucan + H2O | from yeast | Trichoderma harzianum | ? | - |
? | |
| cell wall glucan + H2O | from yeast and different fungi, slight activity, involvement in mycoparasitism | Trichoderma harzianum | reducing sugar + ? | - |
? | |
| laminarin + H2O | specifically hydrolyzes beta-1,3-bonds, final products are glucose, laminaribiose and laminaritetraose | Trichoderma harzianum | D-glucose + ? | - |
? | |
| pachyman + H2O | - |
Trichoderma harzianum | ? | - |
? | |
| periodate-oxidized laminarin + H2O | - |
Trichoderma harzianum | ? | - |
? | |
| pustulan + H2O | beta-1,6-glucan, low activity | Trichoderma harzianum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-beta-1,3-glucanase | - |
Trichoderma harzianum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Trichoderma harzianum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
inactivation temperature | Trichoderma harzianum |
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Release 2023.1 (January 2023)
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