KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
Laminarin | value in mg/ml | Hevea brasiliensis | |
additional information | - |
Laminarin | 1.24, isoform GI | Hevea brasiliensis | |
additional information | - |
Laminarin | 1.33 isoform GII | Hevea brasiliensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
isoform GI, SDS-PAGE, gel filtration | Hevea brasiliensis |
35000 | - |
isoform GII, SDS-PAGE, gel filtration | Hevea brasiliensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hevea brasiliensis | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | isoform GII, glycosylated with glucose and/or mannose residues | Hevea brasiliensis |
Purification (Comment) | Organism |
---|---|
to homogeneity, 2step chromatography | Hevea brasiliensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
latex | two isoforms, GI and GII | Hevea brasiliensis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
isoform GII | Hevea brasiliensis |
0.013 | - |
isoform GI | Hevea brasiliensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
laminarin + H2O | hydrolysis at highest rates by isoform GI, lower hydrolysis by isoform GII, enzyme requires a long run of contiguous beta-1,3-D-glucosidic linkages, where glucosyl units neither substituted nor branched | Hevea brasiliensis | D-glucose + ? | - |
? | |
O-carboxymethyl-pachyman + H2O | faster hydrolysis by isoform GII than by isoform GI, isoform GII may play a role in response to fungal infection | Hevea brasiliensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 32000, isoform GI, SDS-PAGE, gel filtration | Hevea brasiliensis |
monomer | 1 * 35000, isoform GII, SDS-PAGE, gel filtration | Hevea brasiliensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable up to, rapid inactivation above | Hevea brasiliensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
isoform GI | Hevea brasiliensis |
5 | - |
isoform GII | Hevea brasiliensis |