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Literature summary for 3.2.1.39 extracted from

  • Churngchow, N.; Suntaro, A.; Wititsuwannnakul, R.
    beta-1,3-Glucanase isoenzymes from the latex of Hevea brasiliensis (1995), Phytochemistry, 39, 505-509.
No PubMed abstract available
Search for more literature for 3.2.1.39

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 Laminarin value in mg/ml Hevea brasiliensis
additional information
-
 Laminarin 1.24, isoform GI Hevea brasiliensis
additional information
-
 Laminarin 1.33 isoform GII Hevea brasiliensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 isoform GI, SDS-PAGE, gel filtration Hevea brasiliensis
35000
-
 isoform GII, SDS-PAGE, gel filtration Hevea brasiliensis

Organism

Organism UniProt Comment Textmining
Hevea brasiliensis
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein isoform GII, glycosylated with glucose and/or mannose residues Hevea brasiliensis

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 2step chromatography Hevea brasiliensis

Source Tissue

Source Tissue Comment Organism Textmining
latex two isoforms, GI and GII Hevea brasiliensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
 isoform GII Hevea brasiliensis
0.013
-
 isoform GI Hevea brasiliensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
laminarin + H2O hydrolysis at highest rates by isoform GI, lower hydrolysis by isoform GII, enzyme requires a long run of contiguous beta-1,3-D-glucosidic linkages, where glucosyl units neither substituted nor branched Hevea brasiliensis D-glucose + ?
-
 ?
O-carboxymethyl-pachyman + H2O faster hydrolysis by isoform GII than by isoform GI, isoform GII may play a role in response to fungal infection Hevea brasiliensis ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 32000, isoform GI, SDS-PAGE, gel filtration Hevea brasiliensis
monomer 1 * 35000, isoform GII, SDS-PAGE, gel filtration Hevea brasiliensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable up to, rapid inactivation above Hevea brasiliensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 isoform GI Hevea brasiliensis
5
-
 isoform GII Hevea brasiliensis