Any feedback?
Literature summary for 3.2.1.38 extracted from
- Molecular cloning, characterization and in silico analysis of a thermostable beta-glucosidase enzyme from Putranjiva roxburghii with a significant activity for cellobiose (2017), Phytochemistry, 140, 151-165 .
Application
| Application | Comment | Organism |
|---|---|---|
| industry | potentially an important industrial enzyme due to the broad specificity, catalytic efficiency and thermostability | Putranjiva roxburghii |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Putranjiva roxburghii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E173N | mutant enzyme shows no activity | Putranjiva roxburghii |
| E389N | mutant enzyme shows no activity | Putranjiva roxburghii |
| E446N | beta-fucosidase activity is 26.49% compared to wild-type activity, beta-glucosidase activity is 21.37% compared to wild-type activity, beta-galactosidase activity is 15.7% compared to wild-type activity | Putranjiva roxburghii |
| N172A | beta-fucosidase activity is 18.38% compared to wild-type activity, beta-glucosidase activity is 17.65% compared to wild-type activity, beta-galactosidase activity is 13.4% compared to wild-type activity | Putranjiva roxburghii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.46 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 4.6, 37°C, native enzyme | Putranjiva roxburghii |  |
| 0.51 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 4.6, 37°C, recombinant enzyme | Putranjiva roxburghii | |
| 0.53 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 4.6, 37°C, native enzyme | Putranjiva roxburghii | |
| 0.58 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 4.6, 37°C, recombinant enzyme | Putranjiva roxburghii | |
| 0.64 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 4.6, 37°C, native enzyme | Putranjiva roxburghii | |
| 0.68 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 4.6, 37°C, recombinant enzyme | Putranjiva roxburghii | |
| 0.81 | - |
cellobiose | pH 4.6, 37°C, native enzyme | Putranjiva roxburghii | |
| 0.87 | - |
cellobiose | pH 4.6, 37°C, recombinant enzyme | Putranjiva roxburghii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Putranjiva roxburghii | A0A023MIF8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| a single step concanvalin A affinity chromatography for native PRGH1 improves the yield and reduced the purification time | Putranjiva roxburghii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-fucopyranoside + H2O | relative activity of 125% as compared to 4-nitrophenyl beta-D-glucopyranoside. Maximum hydrolytic activity for beta-D-fucopyranoside followed by beta-D-glucopyranoside and 4-nitrophenyl beta-D-galactopyranoside | Putranjiva roxburghii | 4-nitrophenol + beta-D-fucopyranose | - |
? | |
| 4-nitrophenyl beta-D-galactopyranoside + H2O | maximum hydrolytic activity for beta-D-fucopyranoside followed by beta-D-glucopyranoside and 4-nitrophenyl beta-D-galactopyranoside | Putranjiva roxburghii | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | maximum hydrolytic activity for beta-D-fucopyranoside followed by beta-D-glucopyranoside and 4-nitrophenyl beta-D-galactopyranoside | Putranjiva roxburghii | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
| cellobiose + H2O | - |
Putranjiva roxburghii | 2 beta-D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PRGH1 | - |
Putranjiva roxburghii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 65 | native and recombinant enzyme are stable | Putranjiva roxburghii |
| 70 | - |
1 h, the native enzyme retains 67% of its activity while the recombinant enzyme preserves 35% | Putranjiva roxburghii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | - |
native and recombinant enzyme, fucosidase activity | Putranjiva roxburghii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | native and recombinant enzyme are stable over a broad pH range of 4.0-8.0 with maximum retention of their catalytic activity. However, activities decline quickly below pH 4.0 | Putranjiva roxburghii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme may be involved in plant defence | Putranjiva roxburghii |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.99 | - |
cellobiose | pH 4.6, 37°C, recombinant enzyme | Putranjiva roxburghii | |
| 8.57 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 4.6, 37°C, recombinant enzyme | Putranjiva roxburghii | |
| 10.1 | - |
cellobiose | pH 4.6, 37°C, native enzyme | Putranjiva roxburghii | |
| 11.5 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 4.6, 37°C, native enzyme | Putranjiva roxburghii | |
| 14.9 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 4.6, 37°C, recombinant enzyme | Putranjiva roxburghii | |
| 17.9 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 4.6, 37°C, recombinant enzyme | Putranjiva roxburghii | |
| 22.6 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 4.6, 37°C, native enzyme | Putranjiva roxburghii | |
| 32.1 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 4.6, 37°C, native enzyme | Putranjiva roxburghii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
