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Literature summary for 3.2.1.38 extracted from

  • Verhoef, R.; Beldman, G.; Schols, H.A.; Siika-aho, M.; Raettoe, M.; Buchert, J.; Voragen, A.G.
    Characterisation of a 1,4-beta-fucoside hydrolase degrading colanic acid (2005), Carbohydr. Res., 340, 1780-1788.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
colanic acid substrate inhibition at high concentrations Citrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Citrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
337000
-
2 * 337000, SDS-PAGE, the enzyme is part of a high-molecular weight multiprotein complex of at least 6 individual proteins Citrobacter sp.
775000
-
native PAGE, the enzyme is part of a high-molecular weight multiprotein complex of at least 6 individual proteins Citrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
colanic acid + H2O Citrobacter sp. degradation ?
-
?

Organism

Organism UniProt Comment Textmining
Citrobacter sp.
-
VTT E-011941, isolated from a sample of compost, containing paper machine fines, chicken manure, and wood bark
-

Purification (Commentary)

Purification (Comment) Organism
17fold, partially, by anion exchange chromatography, and gel filtration Citrobacter sp.

Reaction

Reaction Comment Organism Reaction ID
colanic acid + H2O = beta-D-fucopyranose + 6-O-AcGlcbeta(1-4)[6-O-pyruvylGalbeta(1-4)GlcAbeta(1-3)Galbeta(1-3)]Fucbeta(1-4)Fucbeta-R reaction mechanism Citrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
partially purified enzyme Citrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
colanic acid + H2O degradation Citrobacter sp. ?
-
?
colanic acid + H2O substrate structure, substrate and product purification and analysis, overview, de-O-acetylation of the substrate increases the activity, while depyruvylation reduces it Citrobacter sp. ?
-
?
de-O-acetylated colanic acid + H2O substrate structure, substrate and product purification and analysis, overview, de-O-acetylation of the substrate increases the activity, while depyruvylation reduces it Citrobacter sp. ?
-
?
de-pyruvylated colanic acid + H2O substrate structure, substrate and product purification and analysis, overview, de-O-acetylation of the substrate increases the activity, while depyruvylation reduces it Citrobacter sp. ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 337000, SDS-PAGE, the enzyme is part of a high-molecular weight multiprotein complex of at least 6 individual proteins Citrobacter sp.

Synonyms

Synonyms Comment Organism
1,4-beta-fucoside hydrolase
-
Citrobacter sp.
colanase
-
Citrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Citrobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80
-
Citrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable up to Citrobacter sp.
50
-
5 h, pH 7.0, 70% remaining activity Citrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Citrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
3 8
-
Citrobacter sp.