Inhibitors | Comment | Organism | Structure |
---|---|---|---|
colanic acid | substrate inhibition at high concentrations | Citrobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Citrobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
337000 | - |
2 * 337000, SDS-PAGE, the enzyme is part of a high-molecular weight multiprotein complex of at least 6 individual proteins | Citrobacter sp. |
775000 | - |
native PAGE, the enzyme is part of a high-molecular weight multiprotein complex of at least 6 individual proteins | Citrobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
colanic acid + H2O | Citrobacter sp. | degradation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter sp. | - |
VTT E-011941, isolated from a sample of compost, containing paper machine fines, chicken manure, and wood bark | - |
Purification (Comment) | Organism |
---|---|
17fold, partially, by anion exchange chromatography, and gel filtration | Citrobacter sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
colanic acid + H2O = beta-D-fucopyranose + 6-O-AcGlcbeta(1-4)[6-O-pyruvylGalbeta(1-4)GlcAbeta(1-3)Galbeta(1-3)]Fucbeta(1-4)Fucbeta-R | reaction mechanism | Citrobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.002 | - |
partially purified enzyme | Citrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
colanic acid + H2O | degradation | Citrobacter sp. | ? | - |
? | |
colanic acid + H2O | substrate structure, substrate and product purification and analysis, overview, de-O-acetylation of the substrate increases the activity, while depyruvylation reduces it | Citrobacter sp. | ? | - |
? | |
de-O-acetylated colanic acid + H2O | substrate structure, substrate and product purification and analysis, overview, de-O-acetylation of the substrate increases the activity, while depyruvylation reduces it | Citrobacter sp. | ? | - |
? | |
de-pyruvylated colanic acid + H2O | substrate structure, substrate and product purification and analysis, overview, de-O-acetylation of the substrate increases the activity, while depyruvylation reduces it | Citrobacter sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 337000, SDS-PAGE, the enzyme is part of a high-molecular weight multiprotein complex of at least 6 individual proteins | Citrobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
1,4-beta-fucoside hydrolase | - |
Citrobacter sp. |
colanase | - |
Citrobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Citrobacter sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | - |
Citrobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to | Citrobacter sp. |
50 | - |
5 h, pH 7.0, 70% remaining activity | Citrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Citrobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | - |
Citrobacter sp. |