Literature summary for 3.2.1.38 extracted from

Chinchetru, M.A.; Cabezas, J.A.; Calvo, P.
Characterization and kinetics of beta-D-gluco/fuco/galactosidase from sheep liver (1983), Comp. Biochem. Physiol. B, 75, 719-728.

Search for more literature for 3.2.1.38

General Stability

General Stability	Organism
stable against repeated freezing and thawing cycles, activity unstable in SDS	Ovis aries aries

Inhibitors

Inhibitors	Comment	Organism	Structure
4-O-alpha-D-glucopyranosyl-D-glucose	effective inhibitor at 50 mM	Ovis aries aries
beta-D-fucose	strong competitive inhibition	Ovis aries aries
beta-D-galactose	competitive inhibition	Ovis aries aries
beta-D-glucose	weak competitive inhibition	Ovis aries aries
D-fucono-gamma-lactone	effective inhibition at 50 mM	Ovis aries aries
glucono 1,5-lactone	effective inhibition at 50 mM	Ovis aries aries
Hg2+	effective inhibitor at 10 mM	Ovis aries aries
KCN	effective inhibitor at 10 mM	Ovis aries aries
Tris	effective inhibitor at 10 mM	Ovis aries aries
Urea	lowers beta-D-glucosidase and beta-D-fucosidase activities, not beta-D-galactosidase activity	Ovis aries aries

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.2	-	p-nitrophenyl-beta-D-glucoside	-	Ovis aries aries
0.25	-	p-nitrophenyl-beta-D-galactoside	-	Ovis aries aries
0.33	-	p-nitrophenyl-beta-D-fucoside	-	Ovis aries aries

Organism

Organism	UniProt	Comment	Textmining
Ovis aries aries	-	sheep	-

Purification (Commentary)

Purification (Comment)	Organism
to homogeneity by several chromatography steps, isoelectric focusing	Ovis aries aries

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Ovis aries aries	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
p-nitrophenyl-beta-D-fucoside + H2O	common active site for beta-D-fucosidase and beta-D-glucosidase, transglycosylation activity, independent active site for beta-D-galactosidase	Ovis aries aries	p-nitrophenol + beta-D-fucose	-	?
p-nitrophenyl-beta-D-fucoside + H2O	contains a single active site for hydrolysis of both p-nitrophenyl-beta-D-fucoside and p-nitrophenyl-beta-D-glucoside	Ovis aries aries	p-nitrophenol + beta-D-fucose	-	?
p-nitrophenyl-beta-D-galactoside + H2O	-	Ovis aries aries	p-nitrophenol + beta-D-galactose	-	?
p-nitrophenyl-beta-D-glucoside + H2O	contains a single active site for hydrolysis of both p-nitrophenyl-beta-D-glucoside and p-nitrophenyl-beta-D-fucoside	Ovis aries aries	p-nitrophenol + beta-D-glucose	-	?

Synonyms

Synonyms	Comment	Organism
beta-D-gluco/fuco/galactosidase	-	Ovis aries aries

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
40	-	not stable above	Ovis aries aries

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.5	5.5	-	Ovis aries aries

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5.5	7	beta-D-fucosidase, beta-D-glucosidase, beta-D-galactosidase activity	Ovis aries aries

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Release 2023.1 (January 2023)