Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoCl2 | 10 mM, slight inhibition | Aspergillus niger | |
CuSO4 | 10 mM, 71.34% inhibition | Aspergillus niger | |
MgSO4 | 10 mM, slight inhibition | Aspergillus niger | |
MnSO4 | 10 mM, slight inhibition | Aspergillus niger | |
Sodium azide | 10 mM, 5-6% inhibition | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.17 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 4.0, 65°C | Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | addition of 10 mM CaCl2 enhances the beta-xylosidase activity by 10.22% | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120480 | - |
SDS-PAGE | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger ADH-11 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.09 | - |
substrate: 4-nitrophenyl alpha-L-arabinofuranoside, pH 4.0, 65°C | Aspergillus niger |
5.4 | - |
substrate: birchwood xylan, pH 4.0, 65°C | Aspergillus niger |
31.5 | - |
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 4.0, 65°C | Aspergillus niger |
61.17 | - |
substrate: 4-nitrophenyl beta-D-xylopyranoside, pH 4.0, 65°C | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger ADH-11 | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus niger | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus niger ADH-11 | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Aspergillus niger | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Aspergillus niger ADH-11 | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
birchwood xylan + H2O | - |
Aspergillus niger | beta-D-xylose + ? | - |
? | |
birchwood xylan + H2O | - |
Aspergillus niger ADH-11 | beta-D-xylose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 120480, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Aspergillus niger |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | 60°C: 81% of maximal activity, 70°C: 78% of maximal activity | Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
3 h, stable | Aspergillus niger |
75 | - |
51.13% activity is retained after 1 h. 15% activity is retained up to 3 h | Aspergillus niger |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
36.08 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 4.0, 65°C | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 4.5 | pH 3.5: 73% of maximal activity, pH 4.5: 78% of maximal activity | Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
the enzyme retain its activity up to 87.37% at pH 4 after 3 h | Aspergillus niger |
5 | - |
3 h, activity is reduced to 30.21% | Aspergillus niger |
6 | - |
3 h, activity is reduced to 23.07% | Aspergillus niger |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30.84 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 4.0, 65°C | Aspergillus niger |