Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethanol | activity is promoted to 116.6% by a concentration of methanol below 15% | Thermotoga petrophila | |
methanol | activity is promoted to 128.9% by a concentration of methanol below 15% | Thermotoga petrophila |
Application | Comment | Organism |
---|---|---|
pharmacology | cooperated transformation of ginsenoside extract to the pharmacologically active ginsenoside 20(S)-Rg3 with a beta-glucosidase. After transforming under optimal condition, the 20 g/l of ginsenoside extract is transformed into 6.28 g/l of Rg3 within 90 min, with a corresponding molar conversion of 95.0% and Rg3 productivity of 1793.49 mg/l/h, respectively | Thermotoga petrophila |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Thermotoga petrophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, 10% inhibition | Thermotoga petrophila | |
DMSO | - |
Thermotoga petrophila | |
Hg2+ | 1 mM, complete inhibition | Thermotoga petrophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.126 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 6.0, 90°C | Thermotoga petrophila | |
2.684 | - |
4-nitrophenyl alpha-L-arabinopyranoside | pH 6.0, 90°C | Thermotoga petrophila | |
6.165 | - |
4-nitrophenyl alpha-L-arabinofuranoside | pH 6.0, 90°C | Thermotoga petrophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 1 mM, activity increases to 104% of control | Thermotoga petrophila | |
K+ | 1 mM, activity increases to 104% of control | Thermotoga petrophila | |
Mg2+ | 1 mM, activity increases to 105% of control | Thermotoga petrophila | |
Sr2+ | 1 mM, activity increases to 107% of control | Thermotoga petrophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
86700 | - |
SDS-PAGE | Thermotoga petrophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga petrophila | - |
- |
- |
Thermotoga petrophila DSM 13995 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga petrophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
66.94 | - |
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 6.0, 90°C | Thermotoga petrophila |
223.14 | - |
substrate: 4-nitrophenyl alpha-L-arabinopyranoside, pH 6.0, 90°C | Thermotoga petrophila |
229.8 | - |
substrate: 4-nitrophenyl beta-D-xylopyranoside, pH 6.0, 90°C | Thermotoga petrophila |
312.84 | - |
substrate: 4-nitrophenyl alpha-L-arabinofuranoside, pH 6.0, 90°C | Thermotoga petrophila |
343.53 | - |
substrate: beta-1,4-D-xylobiose, pH 6.0, 90°C | Thermotoga petrophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Thermotoga petrophila | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Thermotoga petrophila DSM 13995 | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl alpha-L-arabinopyranoside + H2O | - |
Thermotoga petrophila | 4-nitrophenol + alpha-L-arabinopyranose | - |
? | |
4-nitrophenyl alpha-L-arabinopyranoside + H2O | - |
Thermotoga petrophila DSM 13995 | 4-nitrophenol + alpha-L-arabinopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermotoga petrophila | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermotoga petrophila DSM 13995 | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Thermotoga petrophila | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Thermotoga petrophila DSM 13995 | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
beta-1,4-D-xylobiose + H2O | - |
Thermotoga petrophila | beta-D-xylose | - |
? | |
ginsenoside Rb2 + H2O | - |
Thermotoga petrophila | ginsenoside Rd + alpha-L-arabinopyranose | - |
? | |
ginsenoside Rc + H2O | - |
Thermotoga petrophila | ginsenoside Rd + alpha-L-arabinofuranose | - |
? | |
additional information | no activity with 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-L-rhamnopyranoside, 2-nitrophenyl beta-D-glucopyranoside | Thermotoga petrophila | ? | - |
? | |
additional information | no activity with 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-L-rhamnopyranoside, 2-nitrophenyl beta-D-glucopyranoside | Thermotoga petrophila DSM 13995 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 86700, SDS-PAGE | Thermotoga petrophila |
Synonyms | Comment | Organism |
---|---|---|
Tpexyl3 | - |
Thermotoga petrophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Thermotoga petrophila |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | 100 | activity is more than 50% of the maximum activity in the temperature range from 75°C to 100°C | Thermotoga petrophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
residual activity is more than 85% of its original activity after being incubated at 70°C for 2 h | Thermotoga petrophila |
90 | - |
residual activity is more than 50% of its original activity after being incubated at 90°C for 2 h | Thermotoga petrophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
982.321 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 6.0, 90°C | Thermotoga petrophila | |
1000.73 | - |
4-nitrophenyl alpha-L-arabinopyranoside | pH 6.0, 90°C | Thermotoga petrophila | |
2201.64 | - |
4-nitrophenyl alpha-L-arabinofuranoside | pH 6.0, 90°C | Thermotoga petrophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thermotoga petrophila |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7 | the enzyme retains over 60% of its optimum pH activity between pH 4.5 and pH 7.0 | Thermotoga petrophila |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 7.5 | stable within a pH range of 4.0-7.5. The residual beta-xylosidase activity is more than 50% of the original purified enzyme activity after 1 h of incubation at 75 °C in the pH range from 4.5 to 7.5 at the absence of the substrate | Thermotoga petrophila |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
373.25 | - |
4-nitrophenyl alpha-L-arabinopyranoside | pH 6.0, 90°C | Thermotoga petrophila | |
457.9 | - |
4-nitrophenyl alpha-L-arabinofuranoside | pH 6.0, 90°C | Thermotoga petrophila | |
7808.73 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 6.0, 90°C | Thermotoga petrophila |