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Literature summary for 3.2.1.37 extracted from

  • Terrasan, C.; Aragon, C.; Masui, D.; Pessela, B.; Fernandez-Lorente, G.; Carmona, E.; Guisan, J.
    beta-Xylosidase from Selenomonas ruminantium Immobilization, stabilization, and application for xylooligosaccharide hydrolysis (2016), Biocatal. Biotransform., 34, 161-171 .
No PubMed abstract available
Search for more literature for 3.2.1.37

General Stability

General Stability Organism
coating the enzyme with aldehyde-dextran or polyethylenimine, fully stabilizes the quaternary structure of the enzyme rendering much more stabilization to the biocatalyst. The enzyme coated with polyethylenimine of molecular weight 1300 is the most stable one exhibiting an half-life of more than 10 days at pH 5.0 and 50°C, being 240fold more stable than the free enzyme Selenomonas ruminantium

Inhibitors

Inhibitors Comment Organism Structure
D-glucose
-
 Selenomonas ruminantium
D-xylose
-
 Selenomonas ruminantium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
 4-nitrophenyl beta-D-xylopyranoside pH and temperature not specified in the publication Selenomonas ruminantium

Organism

Organism UniProt Comment Textmining
Selenomonas ruminantium
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Selenomonas ruminantium 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Selenomonas ruminantium 4-nitrophenol + beta-D-xylopyranose
-
 ?

Subunits

Subunits Comment Organism
homotetramer
-
 Selenomonas ruminantium

Synonyms

Synonyms Comment Organism
beta-xylosidase
-
 Selenomonas ruminantium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 enzyme coated with polyethylenimine shows optimum activity at 55°C Selenomonas ruminantium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.82
-
 4-nitrophenyl beta-D-xylopyranoside pH and temperature not specified in the publication Selenomonas ruminantium

pH Range

pH Minimum pH Maximum Comment Organism
4 6 enzyme coated with polyethylenimine shows optimum activity in the pH range 4.0–6.0 Selenomonas ruminantium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.82
-
 4-nitrophenyl beta-D-xylopyranoside pH and temperature not specified in the publication Selenomonas ruminantium