Application | Comment | Organism |
---|---|---|
biofuel production | the beta-xylosidase when combined with xylanase shows positive effect on xylan hydrolysis. The higher amount of glucose and xylose generated from sugarcane bagasse hydrolysis using the commercial cocktail Multifect CL supplemented with beta-xylosidase demonstrates that this enzyme has potential to be used as a supplement for commercial cocktails to improve the yield of xylose and glucose release, and this is of great importance for the production of second generation ethanol | Ceratocystis fimbriata |
biotechnology | the beta-xylosidase when combined with xylanase shows positive effect on xylan hydrolysis. The higher amount of glucose and xylose generated from sugarcane bagasse hydrolysis using the commercial cocktail Multifect CL supplemented with beta-xylosidase demonstrates that this enzyme has potential to be used as a supplement for commercial cocktails to improve the yield of xylose and glucose release, and this is of great importance for the production of second generation ethanol | Ceratocystis fimbriata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AlCl3 | about 85% residual activity at 1 mM | Ceratocystis fimbriata | |
CuSO4 | about 70% residual activity at 1 mM | Ceratocystis fimbriata | |
D-xylose | competitive inhibition, about 65% residual activity at 1 mM | Ceratocystis fimbriata | |
FeSO4 | about 65% residual activity at 1 mM | Ceratocystis fimbriata | |
additional information | not inhibited by CoCl2, KCl, MgSO4, CaCl2, EDTA, D-glucose, arabinose, and fructose | Ceratocystis fimbriata | |
SDS | about 82% residual activity at 1 mM | Ceratocystis fimbriata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.326 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 5.0, 50°C | Ceratocystis fimbriata | |
0.326 | - |
4-nitrophenyl beta-D-xylopyranoside | at pH 5.0 and 50°C | Ceratocystis fimbriata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Ceratocystis fimbriata | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
161600 | - |
SDS-PAGE | Ceratocystis fimbriata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ceratocystis fimbriata | - |
- |
- |
Ceratocystis fimbriata RM 35 | - |
- |
- |
Ceratocystis fimbriata RM35 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Ceratocystis fimbriata |
DEAE-Sepharose column chromatography and Sephacryl S-300 gel filtration | Ceratocystis fimbriata |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.014 | - |
substrate: 4-nitrophenyl beta-D-glucoside, pH 5.0, 50°C | Ceratocystis fimbriata |
0.126 | - |
substrate: 4-nitrophenyl beta-D-xylopyranoside, pH 5.0, 50°C | Ceratocystis fimbriata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | 11.1% activity compared to beta-D-xylopyranoside | Ceratocystis fimbriata | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | 11.1% activity compared to beta-D-xylopyranoside | Ceratocystis fimbriata RM35 | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | 11.1% activity compared to beta-D-xylopyranoside | Ceratocystis fimbriata RM 35 | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | activity is 11.1% compared to activity with 4-nitrophenyl-beta-D-xylopyranoside. The enzyme shows selectivity for beta configuration | Ceratocystis fimbriata | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | activity is 11.1% compared to activity with 4-nitrophenyl-beta-D-xylopyranoside. The enzyme shows selectivity for beta configuration | Ceratocystis fimbriata RM35 | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | activity is 11.1% compared to activity with 4-nitrophenyl-beta-D-xylopyranoside. The enzyme shows selectivity for beta configuration | Ceratocystis fimbriata RM 35 | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | 100% activity | Ceratocystis fimbriata | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | the enzyme shows selectivity for beta configuration | Ceratocystis fimbriata | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | 100% activity | Ceratocystis fimbriata RM35 | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | the enzyme shows selectivity for beta configuration | Ceratocystis fimbriata RM35 | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | 100% activity | Ceratocystis fimbriata RM 35 | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | the enzyme shows selectivity for beta configuration | Ceratocystis fimbriata RM 35 | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
additional information | no activity with 4-nitrophenyl alpha -L-arabinopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl alpha-D-mannopyranoside, or 4-nitrophenyl beta-D-mannopyranoside | Ceratocystis fimbriata | ? | - |
? | |
additional information | no activity with 4-nitrophenyl alpha -L-arabinopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl alpha-D-mannopyranoside, or 4-nitrophenyl beta-D-mannopyranoside | Ceratocystis fimbriata RM35 | ? | - |
? | |
additional information | no activity with 4-nitrophenyl alpha -L-arabinopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl alpha-D-mannopyranoside, or 4-nitrophenyl beta-D-mannopyranoside | Ceratocystis fimbriata RM 35 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 161600, SDS-PAGE | Ceratocystis fimbriata |
Synonyms | Comment | Organism |
---|---|---|
beta-xylosidase | - |
Ceratocystis fimbriata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Ceratocystis fimbriata |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | significant enzyme activity in the range of 5565 °C.At 50°C and 70°C, the enzyme activity is reduced, reaching about 40% and 50% of its maximum activity, respectively. Considerable inactivation is detected below 35°C and above 75°C | Ceratocystis fimbriata |
50 | 70 | 50°C: about 40% of maximal activity, 70°C: about 50% of maximal activity | Ceratocystis fimbriata |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
1 h, 14% loss of activity | Ceratocystis fimbriata |
60 | - |
24 h, 30% loss of activity | Ceratocystis fimbriata |
60 | - |
the enzyme maintains 70% of its maximum activity after 24 h of incubation at 60°C | Ceratocystis fimbriata |
65 | - |
30 min, 48% loss of activity, 1 h, 47% loss of activity | Ceratocystis fimbriata |
70 | - |
5 min, 74% loss of activity, Completely inactivated after 1 h | Ceratocystis fimbriata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.9 | - |
- |
Ceratocystis fimbriata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.8 | 5 | the enzyme retains more than 90% of its maximum activity at a pH range from pH 3.8 to pH 4.3 at 50°C. At pH 5.0 the enzyme maintains about 60% of its maximal activity | Ceratocystis fimbriata |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.9 | - |
pH 5.0, room temperature, 12 h, the enzyme maintains 73.1% of its maximal activity | Ceratocystis fimbriata |
2.9 | 8 | the enzyme maintains significant activity when pre-incubated at pH from 2.9 to 8.0 for 12 h, with 73.1% and 51.5% of its maximum activity, respectively | Ceratocystis fimbriata |
8 | - |
pH 5.0, room temperature, 12 h, the enzyme maintains 51.1% of its maximal activity | Ceratocystis fimbriata |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.79 | - |
D-xylose | pH 5.0, 50°C | Ceratocystis fimbriata | |
4.79 | - |
D-xylose | at pH 5.0 and 50°C | Ceratocystis fimbriata |
General Information | Comment | Organism |
---|---|---|
metabolism | beta-xylosidase combined with a xylanase had an additive effect on beechwood xylan hydrolysis, generating xylose as a final product | Ceratocystis fimbriata |