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Literature summary for 3.2.1.37 extracted from

  • Mhetras, N.; Liddell, S.; Gokhale, D.
    Purification and characterization of an extracellular beta-xylosidase from Pseudozyma hubeiensis NCIM 3574 (PhXyl), an unexplored yeast (2016), AMB Express, 6, 73 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.37

Activating Compound

Activating Compound Comment Organism Structure
ethanol ethanol at 5, 10 and 15% (v/v) concentration enhances the enzyme activity Pseudozyma hubeiensis
FeSO4 about 121.72% activity at 1 mM Pseudozyma hubeiensis

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide strong inhibition, 69% residual activity at 200 mM Pseudozyma hubeiensis
5-bromosuccinimide strong inhibition, 84.7% complete inhibition at 0.1 mM Pseudozyma hubeiensis
Ag+ 93.31% residual activity at 10 mM Pseudozyma hubeiensis
Ca2+ 84.91% residual activity at 10 mM Pseudozyma hubeiensis
Co2+ 74.96% residual activity at 10 mM Pseudozyma hubeiensis
Cu2+ 89.26% residual activity at 10 mM Pseudozyma hubeiensis
D-xylose competitive inhibition Pseudozyma hubeiensis
EDTA 73.09% residual activity at 10 mM Pseudozyma hubeiensis
Hg2+ 86.23% residual activity at 0.1 mM Pseudozyma hubeiensis
Mg2+ 88.47% residual activity at 10 mM Pseudozyma hubeiensis
Mn2+ 89.52% residual activity at 10 mM Pseudozyma hubeiensis
additional information not inhibited by diethyl dicarbonate, phenylmethyl sulfonide, phenyl-glyoxal, N-ethylmaleimide, iodoacetate, citraconic anhydride, and trinotrobenzene sulfonic acid. Ethanol has no effect on enzyme activity at 20% (v/v). Pseudozyma hubeiensis
N-Acetylimidazole strong inhibition, 62% residual activity at 50 mM Pseudozyma hubeiensis
Ni2+ 84.7% residual activity at 10 mM Pseudozyma hubeiensis
Pb2+ 89.22% residual activity at 0.1 mM Pseudozyma hubeiensis
Zn2+ 91.75% residual activity at 10 mM Pseudozyma hubeiensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.537
-
 4-nitrophenyl beta-D-xylopyranoside at pH 4.5 and 60°C Pseudozyma hubeiensis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ about 110.32% activity at 0.1 mM Pseudozyma hubeiensis

Organism

Organism UniProt Comment Textmining
Pseudozyma hubeiensis
-
-
-
Pseudozyma hubeiensis NCIM 3574
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, QAE-Sephadex A50 ion exchange column chromatography, and Superdex 200 gel filtration Pseudozyma hubeiensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-xylopyranoside + H2O highest activity Pseudozyma hubeiensis 4-nitrophenol + beta-D-xylopyranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O highest activity Pseudozyma hubeiensis NCIM 3574 4-nitrophenol + beta-D-xylopyranose
-
 ?
additional information no activity with 4-nitrophenyl beta-glucopyranoside and 4-nitrophenyl alpha-L-arabinopyranoside Pseudozyma hubeiensis ?
-
 ?
additional information no activity with 4-nitrophenyl beta-glucopyranoside and 4-nitrophenyl alpha-L-arabinopyranoside Pseudozyma hubeiensis NCIM 3574 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 2 * 110000, SDS-PAGE Pseudozyma hubeiensis
monomer 2 * 112300, MALDI-TOF mass spectrometry Pseudozyma hubeiensis

Synonyms

Synonyms Comment Organism
beta-xylosidase
-
 Pseudozyma hubeiensis
Xyl
-
 Pseudozyma hubeiensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Pseudozyma hubeiensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 70 about 60% activity at 50°C, 100% activity at 60°C, about 90% activity at 70°C, about 10% activity at 75°C Pseudozyma hubeiensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 the enzyme is stable at 50°C for 4 h Pseudozyma hubeiensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Pseudozyma hubeiensis

pH Range

pH Minimum pH Maximum Comment Organism
3 6 about 60% activity at pH 3.0, about 80% activity at pH 4.0, 100% activity at pH 4.5, about 90% activity at pH 5.0, about 45% activity at pH 6.0 Pseudozyma hubeiensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 the enzyme is stable at pH range 4.0-9.0 for 4 h and retains 75 and 100% activity at pH 3.0 and 9.0, respectively, after incubation for 24 h Pseudozyma hubeiensis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
75
-
 at pH 4.5 and 60°C Pseudozyma hubeiensis D-xylose